self etching primers, due to ease of manipulation, have been extensively used in recent years. these self etching primers containing a relatively weak acid, may not provide an optimal bond strength. the purpose of the present in-vitro investigation was to evaluate the effects of 37% phosphoric acid application prior to applying self etching primers on composite bond strength. in this experiment...

Sequence Independent Single Primer Amplification is one of the most widely used random amplification approaches in virology for sequencing template preparation. This technique relies on oligonucleotides consisting of a 3' random part used to prime complementary DNA synthesis and a 5' defined tag sequence for subsequent amplification. Recently, this amplification method was combined with next ge...

We have assayed genetic polymorphisms in several species of parasitic protozoa by means of random amplified polymorphic DNA (RAPD). One goal was to ascertain the suitability of RAPD markers for investigating genetic and evolutionary problems, particularly in organisms, such as the parasitic protozoa, unsuitable for traditional methods of genetic analysis. Another goal was to test certain hypoth...

The present study was conducted with the objective of finding out the concordance between morphological data and molecular data of Mentha spicata and Mentha royleana. Moreover the study was aimed to investigate the relationship between and within the two species. Mentha species were collected from QAU, Shadara, Chattar, Donga gali and Qarshi industries, Hattar. For molecular study Random Amplif...

aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as selex. in the amplification and regeneration step of selex technique, dsdna is conversed to ssdna. asymmetric pcr is one of the methods used for the generation of ssdna. the purpose of this study was to design a random dna library for selection of aptamers wit...

understanding genetic structure and status of genetic variation of fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. random amplified polymorphic dna (rapd-pcr) was used to study the genetic variation of f. hepatica in sheep and cattle. dna was extracted from adult helminthes removed from livers of each infected ani...

A technique that can be used to isolate vector/insert junctions from clones in vectors, such as yeast artificial chromosomes and P1s, and to sequence plasmid inserts more rapidly has been developed. A vector primer is combined with single, randomly chosen oligonucleotides in PCRs, to create pools of products. With 12-24 random primers used in separate reactions, a given insert junction can freq...

Turkey has very rich quince genetic resources, and the country currently dominates world quince production. In particular, the northeastern part of the country has notable Cydonia oblonga Mill. germplasm. Authenticating the identity of germplasm resources of C. oblonga Mill. would be of great value for breeding practices. In the present study, genetic variations of 14 C. oblonga Mill. genotypes...

Several circular oligonucleotides were synthesized and characterized by electrospray ionization mass spectrometry. Experiments on termination of primer extension catalysed by DNA polymerases, Klenow fragment and Tth have demonstrated that a double helix forming circular 2'-deoxyribooligomer containing a 25mer sequence complementary to the target single-stranded DNA along with a 34mer random mis...