in order to investigate the presence of carnation etched ring virus (cerv), samples of carnation (dianthus caryophyllus), sweet william (dianthus barbatus) and weed plants showing symptoms from greenhouses of khorasan razavi and northern khorasan provinces were collected in autumn 2009. polyclonal antiserum was used in order to detection of cerv by double- antibody sandwich-enzyme linked immuno...

Objective(s): Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity.   Materials and Methods: The antigenic region of V...

Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long...

Turnip crinkle virus (TCV), a member of the genus carmovirus of the Tombusviridae family, has a genome consisting of a single positive-sense RNA molecule that is encapsidated in an icosahedral particle composed of 180 copies of a single type of coat protein. We have employed the CPMV-HT transient expression system to investigate the formation of TCV-like particles following the expression of th...

background: recombinant vaccine technology is one of the most developed means in controlling infectious diseases. however, an effective vaccine against shigella is still missing. objective: to evaluate recombinant ipac protein of shigella as a vaccine candidate. methods: in this study we cloned ipac gene into an expression vector in prokaryotic system. the protein expression was evaluated by sd...

background : amelogenins are the major components of enamel matrix proteins. enamel matrix derivatives (emd) can be used in periodontal diseases to regenerate periodontal tissues. the main aim of this study was to evaluate ex-pression of full-length functional recombinant human amelogenin (rham) in iranian lizard leishmania (i.l.l.) as an alternative eukaryotic expression system. methods : huma...

Abstract Background Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There little information on molecular properties in Kenya, hence we investigated population and genetic diversity Kenya. Methods samples virus-like symptoms were obtained from farmers’ field across country 2018 Illumina sequencing undertaken to de...

Regarding high potential of green plants for development of recombinant vaccines, this research was conducted to evaluate expression of a novel recombinant vaccines against Foot and Mouth Disease (FMDV) in tobacco plant. For this purpose, a synthetic gene encoding 129-169 amino acids of foot and mouth disease virus capsid protein VP1 was transferred to tobacco plant via Agrobacterium-mediated g...