A PCR-based ‘molecular tool box’, based on a region of the ras-related protein gene Ypt 1, was developed for the identification of 15 Phytophthora species that damage forests and trees: P. cactorum , P. cambivora , P. cinnamomi , P. citricola , P. europaea , P. inundata , P. lateralis , P. megasperma , P. nemorosa , P. kernoviae , P. pseudosyringae , P. psychrophila , P. quercina , P. ramorum a...

The aims of this study were to isolate and identify of methicillin resistant Staphylococcus aureus (MRSA) from mastitic cow milk. The investigation presence of mecA genes and molecular characteristics of these isolates using eight primers of repetitive element palindromic-PCR (Rep-PCR) analysis were achieved. Ninety six milk samples, collected from clinical mastitic cows from Sharkia Governorat...

Quasispecies variants and recombination were studied longitudinally in an emergent outbreak of beak and feather disease virus (BFDV) infection in the orange-bellied parrot (Neophema chrysogaster). Detailed health monitoring and the small population size (<300 individuals) of this critically endangered bird provided an opportunity to longitudinally track viral replication and mutation events occ...

Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was perform...

introduction: p. aeruginosa is an important multi-drug resistant (mdr) opportunistic pathogen in hospital-acquired infections. several methods based on dna analysis have been used for investigating genetic diversity of p. aeruginosa isolates. among them, rep‑pcr typing has been introduced as a rapid, low cost method with high discriminatory power. the aims of this study were to investigate geno...

objectives : optimum viewing conditions facilitate identification of radiographic details and decrease the need for retakes, patients’ costs and radiation dose. this study sought to evaluate the effects of different viewing conditions on radiographic interpretation. materials and methods: this diagnostic study was performed by evaluating radiograph of a 7mm-thick aluminum block, in which 10 hol...

in this research, the effect of micro turbulence on the flotation rate of quartz particles was investigated. the maximum particle reynolds number (rep) was obtained at 60.25 with a particle size of -500+420 µm, impeller speed of 900 rpm, bubble surface area flux of 10.21 1/s and micro scale turbulence size of 162 µm. when the micro turbulence size was equal to the particle size, the maximum flo...