abstract in vitro leaf explants of gf677 hybrid rootstock and rabi cultivar were placed on mql medium supplemented with bap at 0, 1, 2, 3 and 4 mg/l and naa at 0, 0.15 and 0.3 mg/l concentrations. the results revealed that bap, naa, genotype and their interactions had significant effect on both percentage and mean number of the regenerated shoots. for gf677, the highest percentage of shoot rege...

The present work describes a novel neoformation process of Brassica juncea (L.) Czern. seedlings from transverse thin cell layers in the presence of CdCl2. In order to investigate the regeneration ability of this crop, the effect of CdCl2 on shoot regeneration (frequency of regeneration and bud number per tTCL) was examined. The tTCL explants were excised from cotyledon, petiole and hypocotyl o...

WHO recommends artemisinin-based combination therapies (ACTs) as the most effective choice to treat malaria. For developing transgenic plants with high accumulation of artemisinin (by introducing genes encoding enzymes which regulate the biosynthetic pathway of artemisinin), an efficient protocol for tissue culture and plant regeneration is necessary. In the present study, leaf explants of Arte...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

Nitraria schoberi (Ghar-e-Daghin in Persian) from Zygophyllaceae (Nitrariaceae) family is considered as a wonderful exquisite plant in terms of its economical, medicinal, animal feed, significant values and also its unique characteristics of being tolerant to the soil salinity, drought and erosion. On the other hand, this plant is challenging with severe problems such as seed dormancy, plant gr...

Chrysanthemum indicum plant is a valuable and beneficial herb for human health. In this paper, we present the results of in vitro propagation Da Lat. The showed that MS medium supplemented with 25 g/l sucrose, 9 agar, pH 5.8 was best shoot regeneration growth (shoot height 2.41 - 2.47 cm, 1 shoot/explant). media BA (0.1, 0.5, 1, 1.5, 2 mg/l), Kinetin mg/l ) TDZ mg/l) were unsuitable C. indicum....

An effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ)] at different concentrations (0.5-10 μM) were evaluated as augmented with Murashige and Skoog (MS 1962) medium. Among all the cytokini...

Cotyledonary leaf from 12 days old aseptically grown seedlings were used as explant. explants of two BINA varieties tomato cultured on MS medium containing different concentrations and combinations BAP, Kn IAA for multiple shoot formation via organogenesis. 100% induction was observed when the supplemented with IAA. Earliest within four weeks. The media 1.5 mg/l BAP + 0.5 0.2 found to be best r...

We investigated the effect of Si concentration on shoot regeneration and salinity tolerance of Ajuga multiflora. Addition of Si to the shoot induction medium significantly increased the frequency of shoot induction. The average number of shoots regenerated per explant decreased on the medium containing NaCl alone, while there was less decrease when the shoot induction medium was supplemented wi...