This paper deals with the state-of-the-art information on high-calcium, ASTM Class C fly ash use in cement-based construction materials, such as high-performance concrete, ready-mixed concrete, etc. The major topics included are: properties of fly ash, effects of fly ash inclusion on fresh and hardened concrete, and future research needs. The fresh concrete properties include workability, water...

The micro-texture of fine and coarse aggregates used on roads is one the main factors responsible for skid resistance asphalt pavements. However, long-term behavior this property regarding effects traffic climate conditions has been poorly studied. Therefore, in paper, two types aggregates, schist amphibolite, commonly that are subjected to winter maintenance actions high temperatures summer ha...

background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

BACKGROUND Mild solubilization of inclusion bodies has attracted attention in recent days, with an objective to preserve the existing native-like secondary structure of proteins, reduce protein aggregation during refolding and recovering high amount of bioactive proteins from inclusion bodies. RESULTS Here we presented an efficient method for mild solubilization of inclusion bodies by using a...

Cell culture from vials usually includes an open phase performed under laminar flow hoods. Even if disposable and small closed systems are available, at least the first cell culture step after vial thawing, which is a centrifugation, is performed in disposable but open containers. This step is critical as the risk of contamination is high and it also has a direct impact on process timelines. In...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...