We have developed a saturation transfer EPR (ST-EPR) method to measure selectively the rotational dynamics of those lipids that are motionally restricted by integral membrane proteins and have applied this methodology to measure lipid-protein interactions in native sarcoplasmic reticulum (SR) membranes. This analysis involves the measurement of spectral saturation using a series of six stearic ...

Introduction Proton electron double resonance imaging (PEDRI) is a wellestablished method for imaging free radicals in biological samples and small animals [ 11. The EPR resonance of the free radical under study is irradiated during the acquisition of an MR image; the image intensity in parts of the sample containing free radical is altered by the Overhauser effect. In order to reduce the EPR i...

A combination of molecular mechanics (MM), electron paramagnetic resonance spectroscopy (EPR), and spectra simulation (MM-EPR) has been used to determine the solution structures of di- and trinuclear copper(II) complexes of melamine-based oligomacrocyclic ligands. The spin Hamiltonian parameters of the mononuclear, melamine-appended macrocyclic ligand copper(II) complex have been determined by ...

introduction: acute renal failure is a common consequence of sepisis due to concurrent renal ischaemia. the role of nitric oxide (no) in endotoxaemia and in ischaemic injury in the kidney is not well defined. material and methods: in this study we have used an animal model of sepsis induced by injection of bacterial lipopolysaccharide (lps) in the rat and measured renal nitric oxide by x-band e...

Despite the growing interest in the role of reactive oxygen species (ROS) in health and disease, reliable quantitative noninvasive methods for the assessment of oxidative stress in humans are still lacking. EPR technique, coupled to a specific spin probe (CMH: 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine) is here presented as the method of choice to gain a direct measurement of RO...

In standard continuous wave electron paramagnetic resonance (CW-EPR) experiments, the first derivative of absorption lines is detected. This type of a line shape is caused by the magnetic field modulation and is usually an undesired feature, since the sensitivity of CW-EPR drastically decreases with increasing linewidth. A new approach is introduced, which allows for the measurement of absorpti...

Electron Paramagnetic Resonance (EPR) spectroscopy is the method of choice to study paramagnetic cofactors that often play an important role as active centers in electron transfer processes in biological systems. However, in many cases more than one paramagnetic species is contributing to the observed EPR spectrum, making the analysis of individual contributions difficult and in some cases impo...