Introduction: In stroke models, Inducible Nitric Oxide Synthase (iNOS) expression initiates cellular toxicity due to excessive Nitric Oxide (NO) generation. Anchusa italica is a medicinal herb with anti-inflammatory, antioxidant and neuroprotective properties. This study evaluated the antioxidant activity and NOS mRNA expression of the Hydroalcoholic Extract Of Anchusa Italica (HEAI) in an expe...

Messenger RNA (mRNA) has come into the spotlight due to its potential for addressing a staggering number of diseases, while lack effective and safe carriers in vivo delivery significantly limits clinical application. Herein, lipid-like nanoparticles (LLNs) containing three new cholesterol derivatives achieve liver targeting mRNA is investigated. The central composite design (CCD) used tailor fo...

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2 Background 5 Materials and Methods 10 Results 15 Discussion 26 Appendix 31 Bibliography 32 Acknowledgements 35

Two decades ago, mRNA became the focus of research in molecular medicine and was proposed as an active pharmaceutical ingredient for the therapy of cancer. In this regard, mRNA has been mainly used for ex vivo modification of antigen-presenting cells (APCs), such as dendritic cells (DCs). This vaccination strategy has proven to be safe, well tolerated and capable of inducing tumor antigen-speci...