The presence of regional lymph node metastasis is one of the most significant poor-prognosis factors in patients with biliary tract carcinoma. To establish a sensitive reverse transcription (RT)-PCR assay to detect micrometastases in lymph nodes of biliary tract carcinoma, we first investigated the optimal markers in biliary tract carcinoma. The expressions of the six candidates for a suitable ...

during 2009-10, real time rt-pcr and conventional rt-pcr techniques used for detecting btvs rna in 310 blood samples. for real time and gel based rt-pcr segment-1 and segment-10 selected as conserve genes to search any btv strains. using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. sensitivity of both molecular techniques evalua...

the effect of different storage temperatures on senescence and postharvest performance in cut scapes of three iris species (iris germanica, iris reticulata and iris kashmiriana) was studied with the aim to develop a cost-effective storage protocol so as to bring out the transportation of these cut flowers. the scapes were subjected to two different storage treatments-dry storage and wet storage...

Curative treatment for base-of-tongue squamous cell carcinoma (BOT SCC) has evolved over time; however, comparative outcomes analysis for various treatment strategies is lacking. The authors reviewed the evolution of treatment modality and radiotherapy (RT) technique for 231 consecutive BOT SCC patients at our institution between 1981 and 2011. Treatment modalities included definitive chemoradi...

A method based on infection of CaCo-2 cultured cell monolayers (CC) and reverse transcription-PCR (RT-PCR) was developed for the specific detection of infectious astrovirus. The procedure was validated by titrating poliovirus stocks in parallel in CaCo-2 cells by determining the most probable number of cytopathogenic units and by cell culture and subsequent RT-PCR (CC-RT-PCR). CC-RT-PCR was the...

Objective. To investigate the independent and additive effects of resistance training (RT) and soy isoflavone (ISO) on body composition in postmenopausal women (PW). Method. This study used a placebo-controlled, double-blind (soy), randomized (ISO versus placebo) x (RT versus No RT) design. A total of 80 PW, aged 45-70 years, were randomly (71 completed 9-months intervention): RT + ISO (n = 15)...

BACKGROUND The impact of radiotherapy alone (RT-alone) and radiotherapy plus concurrent chemotherapy with cisplatin (RT-CHT) was determined for lymphocyte subpopulations in cervical cancer patients. MATERIALS AND METHODS Thirty-four women were treated either with RT-alone (n=14) or RT-CHT (n=20). Peripheral lymphocytes were collected before, immediately after and 6, 12 and 24 weeks after comp...

Residents of the village Gabahan Rt. 05 Rw. Most Jombor's 12 sub-districts work as farm laborers and hand desperately need additional income to meet needs their families. They have never received counseling or training on organic composting by universities other institutions. From results interview, several residents wanted manufacture compound fertilizer an alternative increase financial incom...