The competitive antagonist a-conotoxin-MII (a-CTx-MII) is highly selective for the a3b2 neuronal nicotinic receptor. Other receptor subunit combinations (a2b2, a4b2, a3b4) are .200fold less sensitive to blockade by this toxin. Using chimeric and mutant subunits, we identified amino acid residues of a3 and b2 that participate in determination of a-CTx-MII sensitivity. Chimeric a subunits, constr...

The development of a low temperature kinetic method for the flash photolysis of the compounds of membrane-bound cytochrome a3 with carbon monoxide in the presence of oxygen affords evidence for three categories of functional intermediate compounds of cytochrome a3 and oxygen. The three classes are identified as follows: Compounds of Type A are considered to be "oxy" compounds of the ferrous hem...

Calystegines were isolated from the root cultures of Physalis divaricata, P. pubescens, P. philadelphia,and P. philadelphia (solanaceae). Calystegines of P. divaricata were identified as calystegine A3, A5, B1 and B2, with concentrations of 6.99, 4.41, 8.52, and 14.7 ?g/g in fresh mass, respectively. Root cultures of P.pubescens contain calystegine A3, B1 and B2, P. philadelphia only contains ...

High-risk human papillomavirus (HR-HPV) infections cause approximately 5% of all human cancers worldwide. These include almost all cervical carcinomas, a leading global cause of cancer deaths, as well as a significant percentage of other anogenital tract cancers and a growing fraction of oropharyngeal carcinomas. To accommodate their life cycles, HR-HPVs need to extensively rewire infected cell...

Annexins are a family of intracellular proteins that bind membrane phospholipids in a Ca2+ concentration-dependent manner and are involved in cellular processes, including apoptosis, proliferation and differentiation. Hypoxia-inducible factor-1α (HIF-1α) has been hypothesized to be critical in the angiogenesis of tumors. We hypothesized that Annexin A3, a member of the Annexin family, and HIF-1...

Factor VIII (fVIII) circulates as a heavy chain/light chain (A1-A2-B/ap-A3-C1-C2) heterodimer. The 41-residue light chain activation peptide, ap, is cleaved from fVIII during proteolytic activation by thrombin or factor Xa. We constructed 7 active recombinant hybrid B-domainless human/porcine fVIII molecules that contained combinations of porcine sequence replacements within the A2, ap-A3, and ...

A bacterial strain Pseudomonas sp. a3 capable of degrading nitrobenzene, phenol, aniline, and other aromatics was isolated and characterized. When nitrobenzene was degraded, the release of NH(4) (+) was detected, but not of NO(2) (-). This result implied that nitrobenzene might have a partial reductive metabolic pathway in strain a3. However, aniline appeared as one of the metabolites during th...

The focus of this paper is to present students’ perceptions of the recently developed A3 method for problem solving in the healthcare environment. The students were all employees of a large healthcare provider and were enrolled in a customized healthcare EMBA Program. Each student in the class was required to complete an individual A3 Project in order to improve a process at the department for ...