The main alkaline phosphatase isoenzymes of human bile have been purified by DEAE-cellulose chromatography. Characteristics of the isoenzymes, such as electrophoretic mobility before and after butanol extraction, Michaelis constant, and change in electrophoretic mobility following exposure to neuraminidase have been studied and compared with isoenzymes from other sources. The results show that ...

Recombinant alkaline phosphatases are becoming promising protein therapeutics to prevent skeletal mineralization defects, inflammatory bowel diseases, and treat acute kidney injury. By substituting the flexible crown domain of human intestinal alkaline phosphatase (IAP) with that of the human placental isozyme (PLAP) we generated a chimeric enzyme (ChimAP) that retains the structural folding of...

An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phosphoamidase, beta-glucosidase, leucine arylamidase, and acetatase and were hemolyt...

Alkaline phosphatases are known to occur in the various body tissues and in the blood serum. It may reasonably be said that the phosphatase of serum is a mixture of the enzymes contributed by the tissues in varying amounts. Under pathological conditions the contribution of phosphatase from particular tissues may be increased enormously. This fact has been utilized as an aid in clinical diagnosi...

The Panax ginseng has been used as traditional medicine for past several years among oriental people. The present investigation has been made to assess the radioprotective efficacy of ginseng root extract in the testicular enzymes of Swiss albino mice. The Swiss albino mice were divided into different groups. (i) Ginseng treated group: The animals were administered 10 mg/kg body weight ginseng ...

For the study of alkaline phosphatase systems a non-phosphate-containing buffer is required. The buffers in common use for the alkaline pH range are ammonia, borate, glytine and veronal. Ammonia and borate have a retarding effect on the rate of ,enzymic hydrolysis of phosphoric esters. Glycine activates phosphatase when present in very low concentrations (0 1-1 mm), but in the concentrations us...