نتایج جستجو برای: alkaline phosphatases
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in this study cellular phosphatase activity, referred to as enzyme developed color (edc) assay, was used for an in vitro assessment of cell proliferation and cytotoxicity. optimum conditions were established and found to be 10 x103 cells / well at 37°c and 60 min incubation (for developing color). under the same conditions a direct correlation between optical density (00) and cell number was ob...
Many tissues contain enzymes which will hydrolyse the organic phosphorus compounds to give free phosphoric acid. These enzymes are known as phosphatases. Some of these phosphatases are most active in an alkaline medium and the others in an acid medium. The phosphatase of bone (Yamane, 1931), bile (King and Armstrong, 1934) and white blood cells (Roche, 1931) are alkaline phosphatases, while tho...
Acid and Alkaline Phosphatases There are probably many different phosphatases present in living organisms and much valuable data is being accumulated on their identity and methods of differential estimation. In the present state of our knowledge, however, and from the aspect of clinical application, we can best think of them in terms of two groups, the socalled' alkaline ' and' acid' phosphatas...
In soil, bioavailable inorganic orthophosphate is found at low concentrations and thus limits biological growth. To overcome this phosphorus scarcity, plants and bacteria secrete numerous enzymes, namely acid and alkaline phosphatases, which cleave orthophosphate from various organic phosphorus substrates. Using profile hidden Markov modeling approaches, we investigated the abundance of various...
The human alkaline phosphatases constitute a system of multiple molecular forms of enzymes in which heterogeneity is partly due to genetic factors and partly to posttranslational modifications. Recognition of the nature and occurrence of these multiple forms has made a significant contribution both to the understanding of changes in alkaline phosphatase values for serum in disease and to the us...
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