Arylamines are widely used in food, drugs, and cosmetics as well as other industries. These chemicals are present ubiquitously in cigarette smoke, smoke emitted from cooking fume hoods as well as are generated by diverse industries. Arylamines can be generated by cleavage of azo dyes by intestinal and skin microbiota. Some arylamines are used as drugs while others are constituents of human meta...

Animal protein plays a key role in the human diet as most balanced amino acid composition; however, its consumption often causes allergic reactions. Plant serves substitute for animal protein. The promising sources of plant are seeds cereals, pulses, oilseeds and cereals. Research aim: selection conditions obtaining isolates enzymatic hydrolysates having desired functional properties from diffe...

The role of maternal allergen exposure in the allergenicity of the offspring remains controversial. Some studies have shown that maternal exposure is a risk factor for allergy in the offspring, whereas other studies have shown that maternal exposure induces immune tolerance and protects offspring from allergy disease. Therefore, we utilized maternal rat allergen exposure model to evaluate the o...

BACKGROUND Red meat allergy presents a novel form of food allergy with severe delayed allergic reactions where IgE antibodies are directed against the carbohydrate α-Gal epitope. Food preparation and processing can influence the allergenicity of proteins. The aim of this study was to characterize the proteomic profile of different beef preparations and to investigate their α-Gal reactivity and ...

Background Double-blind, placebo-controlled food challenge (DBPCFC) is considered the gold standard for food allergy diagnosis. However, this test is rarely performed routinely in clinical practice because of various practical issues, e.g. the lack of a standardized matrix preparation. The aim of this study was to develop and validate a convenient DBPCFC matrix, that can easily be implemented i...

Staphylococcus aureus is a versatile Gram's positive bacterium that can reside as an asymptomatic colonizer, which cause wide range of skin, soft-tissue, and nosocomial infections. A vaccine against multi-drug resistant S. aureus, therefore, urgently needed. Subtractive proteomics reverse vaccinology are newly emerging techniques to design multiepitope-based vaccines. The analysis 7290 proteome...