bainitic transformation at low transformation temperatures leads to a microstructure involving fine plates of bainitic ferrite and thin films of retained austenite. this microstructure has shown ultimate tensile strength of about 2.2 gpa, noticeable uniform elongation in the range of 5 to 30%, hardness values of about 600 to 670 hv and impact toughness in the range of 30 to 40 mpa m1/2. with ca...

I take this opportunity to record some of my own In general, the growth rate of a bacterial strain may be recollections of the work on mutation in the 1940s and within very wide limits independent of the concentration early 1950s that was so influential in the development of a given growth factor; but since at zero concentration of quantitative biology. In 1943, Luria and Delbrück the growth ra...

Larry’s research focuses on biocatalysis and biodegradation. Laboratory studies are centered on microbial catabolic enzymes; complementary informatics projects revolve around the University of Minnesota Biocatalysis/Biodegradation Database, http://umbbd.ahc.umn.edu/ index.html. Industrial microbiology exploits the ecological adaptations that microorganisms have developed to communicate with and...

Since its initial description, the yeast two-hybrid (Y2H) system has been widely used for the detection and analysis of protein-protein interactions. Mating-based strategies have been developed permitting its application for automated proteomic interaction mapping projects using both exhaustive and high-throughput strategies. More recently, a number of prokaryotic two-hybrid (P2H) systems have ...

Adenosine 3':5'-cyclic monophosphate added to exponentially growing cells of Hemophilus influenzae strain Rd increases competence for transformation 100- to 10,000-fold. Cyclic AMP added to near-stationary or stationary cells does not increase competence over the high level normally found in the early stationary phase.

The classic bacterial transformation system, and one of the two most thoroughly studied systems, is Diplococcus pneumoniae. Transformation systems seem to offer a unique opportunity to study, at the molecular level, the hereditary material, deoxyribonucleate, and the molecular units of the organism it determines. Unfortunately this study has been retarded by a lack of well defined biochemical m...

Eukaryotic ribosomal stalk protein L12 and its bacterial orthologue L11 play a central role on ribosomal conformational changes during translocation. Deletion of the two genes encoding L12 in Saccharomyces cerevisiae resulted in a very slow-growth phenotype. Gene RPL12B, but not the RPL12A, cloned in centromeric plasmids fully restored control protein level and the growth rate when expressed in...

Background: Optimization of co-cultivation parameters during Agrobacterium-mediated transformation to Oxalis corniculata evaluated were bacterial density, infection period, acetosyringone (AS) concentration and temperature. Five fold efficiency is achieved in transient gus gene expression after optimizing various parameters. The optimized conditions Agrobacterium tumefaciens growth phase A600nm...

presence of antibiotic resistance markers has always been considered as one of the main safety concerns in transgenic plants and their derived products. elimination of antibiotic selectable markers from transgenics is a major hurdle for finding efficient and safe candidates. herbicide tolerance genes might be attractive alternatives. in this study, a variant form of the 5-enoylpyruvyl shikimate...

Strains of Bacillus subtilis lysogenic for either temperate bacteriophage phi105 or SPO2 were reduced to less than 1.0% of the level of transformation of the nonlysogenic strains. Strains lysogenic for both phi105 and SPO2 are virtually nontransformable, indicating that the effect of lysogeny is additive. Lysogenic cultures transfected at essentially wild-type levels with deoxyribonucleic acid ...