نتایج جستجو برای: bacterial transformation

تعداد نتایج: 422465  

Journal: :international journal of health studies 0
zahra sadri najaf abadi 1 1 dept. of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. farnaz fatemi 1 masoud khosravani 2 2 dept. of medical nanotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran. majid rahmati 3 3 dept. of medical biotechnology, school of medicine, shahroud university of medical sciences, shahroud, iran. esmaeil sadroddiny 1*

background: bone morphogenetic protein 2 (bmp- 2) belongs to the tgf-β superfamily of proteins and plays an important role in the development of bone and cartilage. bmp- 2 is also associated with maintenance and repair of damaged bone. recombinant human bone morphogenetic protein 2 (rhbmp- 2) is now produced by genetic engineering techniques and used in treatment of thin bone fractures in the j...

Paulownia tomentosa (Thunb.) Steud. is a very important hard woody plant, an extremely fast-growing tree and produce timber. Therefore, there is a demand to produce transgenic Paulownia plant resistant to bacterial infection. Microbial infection (especially bacterial one) is serious sever and cause a loss in plant productivity as they bear upon the character and amount of plan...

Journal: :Journal of Bacteriology 1937

ژورنال: دانشور پزشکی 2009
باباشمسی, دکتر محمد , نژادمقدم, محمدرضا , نیاکان, دکتر محمد , هادوی, رضا ,

  Background & Objective: Transformation of plasmid DNA into bacterial competent cells is a key technique for molecular cloning. Transformation can be achieved using either chemical or physical methods, e.g., electroporation. The rate of success in these methods depends on experience and attention to method’s details. Therefore, the higher the efficiency and quality of a transformation method, ...

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

Journal: :Bacteriological reviews 1952
R AUSTRIAN

In reviewing previously the chemical nature and biologic specificity of the substance inducing transformation of pneumococcal types (31), McCarty suggested that the principles involved and the results observed in the study of this reaction have implications beyond those referable to the biology of pneumococcus. The correctness of this point of view has been borne out by the investigations of th...

ژورنال: علوم آب و خاک 2008
بابائیان جلودار, نادعلی , شکیب , علی محمد , نجفی, بهناز, سیفی, علی‌رضا ,

Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...

Journal: :molecular biology research communications 0
maral rahimzadeh department of nanobiotechnology, school of biological sciences, tarbiat modares university, tehran, iran majid sadeghizadeh department of molecular genetics, school of biological sciences, tarbiat modares university, tehran, iran farhood najafi department of resin and additives, institute for color science and technology, tehran, iran shahriar arab department of biophysics, school of biological sciences, tarbiat modares university, tehran, iran hamid mobasheri laboratory of membrane biophysics, institute of biochemistry and biophysics, university of tehran, tehran, iran

cacl2 treatment followed by heat shock is the most common method for artificial transformation. here, the cells were transformed using cacl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. the bl21 strain of escherichia coli (e. coli) was being susceptible using cacl2 treatment. some cells were ...

بابائیان جلودار, نادعلی , شکیب , علی محمد , نجفی, بهناز, سیفی, علی‌رضا ,

Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...

Journal: :Electronic Journal of Biotechnology 2007

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