Chili hotness is very much dependent on the concentration of capsaicin present in the chili fruit. A new biosensor based on a horseradish peroxidase enzyme-capsaicin reaction mediated by ferrocene has been successfully developed for the amperometric determination of chili hotness. The amperometric biosensor is fabricated based on a single-step immobilization of both ferrocene and horseradish pe...

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A health monitoring system (HMS) involving a blood extraction device with a new type of hybrid biosensor comprising an enzyme and a semiconductor has recently been developed. A MOSFET was used as the transducer. The gate electrode was extracted from the MOSFET using a cable. Gold (Au)-plate-immobilized glucose oxidase (Go) was used as a biosensor and attached to the gate electrode. Go was immob...

NiCu alloy nanoparticle-loaded carbon nanofibers (NiCuCNFs) were fabricated by a combination of electrospinning and carbonization methods. A series of characterizations, including SEM, TEM and XRD, were employed to study the NiCuCNFs. The as-prepared NiCuCNFs were then mixed with laccase (Lac) and Nafion to form a novel biosensor. NiCuCNFs successfully achieved the direct electron transfer of L...

A biosensor comprising tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode has been developed. The sensitive element, ie, tyrosinase, was immobilized using a drop-and-dry method followed by cross-linking. Tyrosinase maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrine-quinone, which was electrochemically ...

A sensitive biosensor based on acetylcholinesterase (AChE) and carbon nanosphere (CNS) immobilized on a glassy carbon electrode was developed for the detection of pesticides by the inhibition of AChE activity. The inhibition effect of the pesticides resulted in a decrease of current response of the acetylthiocholine chloride that was used as a substrate to obtain an electrochemical signal. When...

A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range ...

A biosensor was developed for the determination of BOD value of fermentation industry effluent. The developed biosensor was fabricated by immobilizing the microbial consortium on cellulose acetate (CA) membrane in close proximity to a DO probe electrode. The microbial consortium was harvested from the fermentation industry effluent. The BOD biosensor was calibrated by using a solution containin...

The enhanced chemiluminescence (CL) reaction of the luminol±H2O2±horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol±gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obtained for p-iodophenol, p-coumaric acid and 2-naphthol were 0.83 mM, 15 and 48 nM, respectively. Dir...