Carbapenem-resistant Enterobacteriaceae (CRE) has increasingly spread worldwide in the past decade. The prevalence and characteristics of CRE in Thailand are unknown. In this study, we conducted a 2-year surveillance of CRE among 12,741 clinical isolates of Enterobacteriaceae at the largest university hospital in Thailand with molecular characterization of beta-lactamase (bla) genes, including ...

5 Abstract Aim: To analyse the occurrence of bla genes (blaIMP, blaVIM, blaGIM, blaSIM, blaSPM, blaPER, blaKPC, and blaOXA) responsible for carbapenemresistance, as well as the presence of cephalosporin-resistance genes (blaCTX-M, blaTEM, blaSHV and blaampC ) by polymerase chain reactions (PCRs). Methods: The study was performed on routine samples subjected to culture and antibiotics susceptibi...

The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological and phenotypic characteristics of all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection of Antimicrobial Resistance. All confirmed CPE isolates were characterized pheno- and genoty...

Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have emerged as major nosocomial pathogens. bla(KPC), commonly located on Tn4401, is found in Gram-negative bacterial strains, with the two most common variants, bla(KPC-2) and bla(KPC-3), identified in plasmids with diverse genetic backgrounds. In this study, we examined bla(KPC-4)- and bla(KPC-5)-bearing plasmids recovered...

The depletion of blaKPC (K. pneumoniae), blaOXA-50 (P. aeruginosa) and mecA (S. aureus) genes from hospital urines is evaluated to contribute solve the silent pandemic antibiotic-resistance bacteria. A microfluidic flow-through reactor with MMO anode CB/PTFE cathode working at 50 m−2 employed during electrodisinfection photo-electrodisinfection processes. process only achieves an almost negligi...

Methods A 68 year old female who was travelling in Greece was admitted to the ICU of the University Hospital of Ioannina in Greece for urosepsis and hypercapnic coma. The patient was transferred to the ICU of the Spaarne Hospital on September 22 2010 (day 9). Patient was admitted in strict barrier precautions for MRSA. Intestinal carriage of MDR Enterobacteriaceae was screened at admission and ...

Background: Although ceftazidime/avibactam (CAZ/AVI) has become an important option for treating adults and children, no data or recommendations exist neonates. We report a neonatal sepsis case due to CAZ/AVI-resistant blaKPC-2-harboring Klebsiella pneumoniae carrying blaVEB-25 the use of customized active surveillance program in conjunction with enhanced infection control measures. Methods: Th...

The purpose of this study was to detect carbapenemase genes in clinical isolates of carbapenem-resistant Enterobacteriaceae (CRE) obtained from patients admitted to Hua-Hin Hospital, Prachuab Khiri Khan Province, Thailand between January and December 2014. Screening of CRE was initially determined using disk diffusion method, and subsequently using modified Hodge test (MHT). Multiplex PCR was e...

from Iranian patients and the MHT test does not have a high sensitivity for BlaKPC identification in Iran and other countries unless it is confirmed by molecular methods. The Gram-negative bacilli such as Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii are the most important bacteria in nosocomial infection (13). To date, these bacteria are almost multidrug resistant (MDR...