Genetic engineering technology has been successfully used in many plant species, but is limited in woody plants, especially in bamboos. Ma bamboo (Dendrocalamus latiflorus Munro) is one of the most important bamboo species in Asia, and its genetic improvement was largely restricted by the lack of an efficient regeneration and transformation method. Here we reported a plantlet regeneration and A...

SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes have been demonstrated to play a role in somatic embryogenesis in several plant species. As more is learnt about these genes, the view of their role in plant development has broadened. The Medicago truncatula MtSERK1 gene has been associated with somatic embryogenesis and in vitro root formation. In order to study the role of MtSERK1 in de...

The results of studies on in vitro regeneration of chickpea (Cicer arietinum L.) have shown that indirect regeneration via callus was not successful. The introduction of an efficient and repeatable regeneration protocol is necessary for using transgenic technologies for the improvement of chickpea. The present study was undertaken to investigate the possibility of indirect regeneration in vario...

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...

The data article contains the experimental data and images on the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro. Investigated data are related to the research article "Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro" Alsaif et al. (2011) [1] and "Pla...

The already differentiated organs in plants have a remarkable capacity to regenerate new individuals under culture conditions. Plant in vitro regeneration practically starts with the induction of a pluripotent cell mass, the callus, from detached organs on auxin-rich callus-inducing medium (CIM), which is generally required for subsequent regeneration of new bodies. Recent studies show that CIM...

Through observations of smear materials under a dissecting microscope, it was found that small smooth protuberances were produced in regions showing the dry appearance. Trichomes occurred in the regions showing the green color or the white and green color. There were tracheary elements having thickened wall in the inner part of callus, adjacent to which chloroplasts were observed11>. Scanning e...

A protocol for direct shoot regeneration without intervening callus phase was developed by shoot tip culture of MS (murashinge and skoog) medium for teak. The study reveal that aseptically inoculated shoot tip of teak in established shoot induction and shoot multiplication was also observed at same concentration .At BAP 1mg/l, IAA 1mg/l and kin 1mg/l the shoots were weak ,tiny and non separable...

context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replicati...