BACKGROUND Exendin-4 is an incretin mimetic agent approved for type 2 diabetes treatment. However, the required frequent injections restrict its clinical application. Here, the potential use of chitosan-coated poly (d,l-lactide-co-glycolide) (CS-PLGA) nanoparticles was investigated for intestinal delivery of exendin-4. METHODS AND RESULTS Nanoparticles were prepared using a modified water-oil...

Induction of specific humoral and cellular immunity in the lung airways is proposed to be critical for vaccine protection against Mycobacterium tuberculosis (M. tb). To facilitate airway delivery and antigen targeting to the antigen presenting cells in the alveoli, we employed mannosylated chitosan (MCS) to formulate a multi-T-epitope DNA vaccine, pPES, as an intranasal TB vaccine. MCS-DNA nano...

A chitosan-based hydrophilic system containing an olive leaf extract was designed and its antioxidant capacity was evaluated. Encapsulation of olive leaf extract in chitosan microspheres was carried out by a spray-drying process. The particles obtained with this technique were found to be spherical and had a positive surface charge, which is an indicator of mucoadhesiveness. FTIR and X-ray diff...

Chitosan has good biocompatibility, in vivo biodegradability and certain physiological activity, which can be used as a drug carrier to stabilise protect components, causing the promotion of absorption controlling release. Using graphene oxide (GO) cross-linking agent, functional groups its lamellar structure interact effectively with chitosan molecules, leading enhanced mechanical properties o...

Liposomes are commonly used to deliver drugs and contrast agents to their target site in a controlled manner. One of the greatest obstacles in the performance of such delivery vehicles is their stability in the presence of serum. Here, we demonstrate a method to stabilize a class of liposomes that load gadolinium, a magnetic resonance (MR) contrast agent, as a model cargo on their surfaces. We ...

2.4. Sol-Gel Reactions 2070 2.5. Polyol Methods 2071 2.6. Flow Injection Syntheses 2071 2.7. Electrochemical Methods 2071 2.8. Aerosol/Vapor Methods 2071 2.9. Sonolysis 2072 3. Stabilization of Magnetic Particles 2072 3.1. Monomeric Stabilizers 2072 3.1.1. Carboxylates 2073 3.1.2. Phosphates 2073 3.2. Inorganic Materials 2073 3.2.1. Silica 2073 3.2.2. Gold 2074 3.3. Polymer Stabilizers 2074 3.3...

Pickering emulsions (PEs) have recently gained increasing attention as green carriers of bioactive agents due to their surfactant-free and eco-friendly nature. Herein, the effect degree deacetylation (DDA) chitosan on properties chitosan/gum Arabic (CH/GA) particles PEs was investigated. CH/GA with a DDA 96% (high DDA) 78% (low were prepared. The contact angle water drop for prepared high low 8...

Properties of the chitosan films can be improved by incorporating clay minerals. So, solvent-cast β-chitosan containing stevensite-rich or kaolinitic-illitic clays (up to 50 mass %) were characterized for their structural and mechanical properties. The effects molecular weight (MW) deacetylation degree (DD) clay/chitosan ratio on inhibition growth Escherichia coli Staphylococcus aureus studied ...

Intravenous (i.v.) administration of phagocytosable chitin particles (1 to 10 microm) in C57BL/6 mice and SCID mice primed alveolar macrophages (Mphi) within 3 days to yield up to a 50-fold increase in their oxidative burst when elicited in vitro with phorbol myristate acetate (PMA). C57BL/6 mice pretreated with monoclonal antibodies (MAbs) against mouse gamma interferon (IFN-gamma) or NK1.1 sh...

Objective(s): Production of effective tuberculosis (TB) vaccine is necessity. However, the development of new subunit vaccines is faced with concerns about their weak immunogenicity. To overcome such problems, polymers-based vaccine delivery systems have been proposed to be used via various routes. The purpose of this study was to determine the potential of polymeric particles as future vaccine...