An investigation involving seven boars, active in artificial insemination, and 1,350 multiparous sows was conducted at a private farm and aimed at examining the relationship between sperm quality traits and boar fertility in terms of farrowing rate and litter size. This experiment was done for 6 months. The semen samples were evaluated for subjective sperm motility and concentration. Ejaculates...

Chromatin immunoprecipitation, DNase I hypersensitivity and transposase-accessibility assays combined with high-throughput sequencing enable the genome-wide study of chromatin dynamics, transcription factor binding and gene regulation. Although rapidly accumulating publicly available ChIP-seq, DNase-seq and ATAC-seq data are a valuable resource for the systematic investigation of gene regulatio...

Genomes are organized into three-dimensional structures, adopting higher-order conformations inside the micron-sized nuclear spaces (7, 2, 12). Such architectures are not random and involve interactions between gene promoters and regulatory elements (13). The binding of transcription factors to specific regulatory sequences brings about a network of transcription regulation and coordination (1,...

We evaluated the effects of opiate consumption on semen quality, sperm function, seminal plasma antioxidant capacity, and sperm DNA integrity. A total of 142 opiate addict men (group 1) were enrolled in the study and 146 healthy age matched male volunteers (group 2) served as controls. Two semen analyses were performed in all participants. Sperm chromatin structure assay (SCSA) was used to iden...

Central dogma is the most fundamental hypothesis in field of molecular biology and explains genetic information flow from DNA to protein. Beyond residue-by-residue transmission sequential information, chemical modifications DNA, RNA, protein are also relayed course gene expression. Here, this work presents recent evidence supporting bidirectional interplay between chromatin RNA modifications. F...

facioscapulohumeral muscular dystrophy (fshd)is characterized by weakness of the facial, scapular muscles  and the dorsiflexors of the foot. severity in this disorder is highly variable. approximately 95% of individuals with fshd phenotype have type 1 fshd, with d4z4 allele of between one and ten repeat units and about 5% have type 2 fshd with mutations in the chromatin modifier smchd1gene whic...

Background: Acyclovir is asynthetic purin nucleoside analog,which has been reported to be very effective against the treatment of herpes simplex and varicella zoster infections. Acyclovir is known to be toxic to gonads, but available literature fails to provide the indepth analyses of damage.The aim of the present study was to evaluate the effect of Acyclovir on DNA damage and nuclear maturatio...

background: isolating sperms with normal chromatin content is considered vital in intra cytoplasmic sperm injection treatment. today, researchers are trying to find new procedures to select sperms with normal chromatin content and morphology. this study examines the effectiveness of zeta, density gradient centrifugation (pure sperm) and combination of pure- zeta procedures in selecting sperms w...

sperm is produced by the testis and mature in the epididymis. for having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. the effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. in recent years, the fe...