BACKGROUND Hypoxia is a common feature of solid tumors, including HCC. And hypoxia has been reported to play an important role in HCC progression. However, the potential mechanism of miRNAs in hypoxia mediating HCC progression still remains unclear. METHODS The HCC cells were cultured in the atmosphere of 1 % oxygen to induce hypoxia. The microRNA microarray was employed to search for the hyp...

Experiments using live cell 3-color Förster (or fluorescence) resonance energy transfer (FRET) microscopy and corresponding in vitro biochemical reconstitution of the same proteins were conducted to evaluate actin filament nucleation. A novel application of 3-color FRET data is demonstrated, extending the analysis beyond the customary energy-transfer efficiency (E%) calculations. MDCK cells wer...

The number of wetlands in Europe decreased by more than 60% the 1990s compared with beginning twentieth century. Man-made may be an effective way to compensate for loss and degradation freshwater ecosystems. This impacts populations declining duck species, partly due a lack suitable breeding opportunities. In this study, we evaluated productivity invertebrate abundance 13 man-made Finnish that ...

Observations were made of birds and mammals on Adams Island and the southern end of the main Auckland Island between 2 November and 7 December 1989. Thirty-five bird species, fur seals and sealions were observed. Five Auckland Island rail were captured and many more heard on Adams Island. Counts of wandering and royal albatross, yellow-eyed penguins, Auckland Island teal and Auckland Island dot...

Local adaptation frequently occurs across populations as a result of migration-selection balance between divergent selective pressures and gene flow associated with life in heterogeneous landscapes. Studying the effects of selection and gene flow on the adaptation process can be achieved in systems that have recently colonized extreme environments. This study utilizes an endemic South American ...

Many birds, alligators, and turtles spend their entire lifetimes communing between wetlands and adjacent bodies of water, while land animals that normally occupy dry land visit the wetlands to feed. Herons, eagles, sandpipers, ducks, and geese winter in marshes or rest there while migrating. The larvae of shrimp, crab, and other marine animals find shelter in the marsh from larger animals. Blue...

abstract in order to find out a better and cheaper artificial diet for mass rearing of common green lacewing, chrysoperla carnea, an experiment was conducted using completely randomized design (crd) with 4 replications and 5 observations. in this study were used three diets [a mixture of honey, yeast and distilled water (1:1:1), a mixture of honey, yeast plus extract of angomois grain moth eggs...