نتایج جستجو برای: direct sample pcr
تعداد نتایج: 971582 فیلتر نتایج به سال:
Mutations in the epidermal growth factor receptor (EGFR) gene are associated with a favorable clinical response to the EGFR tyrosine kinase inhibitors gefitinib and erlotinib in non-small cell lung cancer (NSCLC). We present here, a new method for the rapid detection of the two most common EGFR mutations (delE746-A750 and L858R) from clinical samples. The methodology involves the combination of...
Abstract Background: Community acquired pneumonia (CAP) is the main cause of mortality and morbidity world wide. Legionella pneumophila is identified as the fourth agent that causes CAP. The aim of this study was to define the prevalence of L. pneumophila among hospitalized children by culture, direct immune-fluorescence, (DFA) and PCR. Methods: In this study 210 sputum samples were collect...
We describe a nested PCR-restriction fragment length polymorphism (RFLP) method for detecting low densities of Cryptosporidium spp. oocysts in natural mineral waters and drinking waters. Oocysts were recovered from seeded 1-liter volumes of mineral water by filtration through polycarbonate membranes and from drinking waters by filtration, immunomagnetizable separation, and filter entrapment, fo...
AIM To compare polymerase chain reaction (PCR) amplification of borrelial DNA and culture isolation of spirochaetes for the diagnosis of Lyme borreliosis by direct detection of Borrelia burgdorferi sensu lato in patients with erythema migrans and acrodermatitis chronica atrophicans lesions. METHODS Skin biopsy specimens from erythema migrans and acrodermatitis chronica atrophicans lesions wer...
background: the aim was to compare three different methods (direct examination, culture and pcr methods) for the diagnosis of acanthamoeba keratitis (ak) in corneal scrapes. methods: twenty eight corneal scrapes and contact lenses were collected from keratitis patients and referred to the department of medical parasitology and mycology, school of public health, tehran university of medical ...
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