Chronic wounds in skin persist because the normal process of wound healing is obstructed. Failures occur in the inflammation and proliferation phases of wound healing that reduce formation of granulation tissue and prevent epithelial migration to close the wound, resulting mostly from vascular insufficiencies of multiple etiologies. Microbial contamination of chronic wounds also contributes imp...

PURPOSE The Ex Vivo Eye Irritation Test (EVEIT) is used to analyse the clinical observations of corneal calcification attributed to the presence of phosphate within applied eye-drops used in treating glaucoma, Still-Chauffard syndrome, ocular burns and dry eyes. METHOD Live corneas from abattoir rabbit eyes were cultured in order to study epithelial healing following mechanical abrasion of th...

PURPOSE The reepithelialization of the corneal surface is an important process for restoring the imaging properties of this tissue. The purpose of the present study was to characterize and validate a new human in vitro three-dimensional corneal wound healing model by studying the expression of basement membrane components and integrin subunits that play important roles during epithelial cell mi...

Tarsorrhaphy is used for the treatment of severe ocular surface disorders and for damage from ocular exposure. The temporary tarsorrhaphy has been shown to aid in the healing of corneal epithelial defects. A variety of temporary techniques have been suggested that allow eyelid closure not only to enable epithelial healing but also to allow access to the eye. We describe a temporary eyelid closu...

Corneal wound healing is a complex process that occurs in response to various injuries and commonly used refractive surgery. It is a significant clinical problem, which may lead to serious complications due to either incomplete (epithelial) or excessive (stromal) healing. Epithelial stem cells clearly play a role in this process, whereas the contribution of stromal and endothelial progenitors i...

Increased leukocyte elastase activity in mice lacking secretory leukocyte protease inhibitor (SLPI) leads to impaired wound healing due to enhanced activity of TGFbeta and perhaps additional mechanisms. Proepithelin (PEPI), an epithelial growth factor, can be converted to epithelins (EPIs) in vivo by unknown mechanisms with unknown consequences. We found that PEPI and EPIs exert opposing activi...

PURPOSE To investigate the effects of insulin and leptin on in vitro wound healing of transformed human corneal epithelial cell monolayers and to identify cellular (migration versus proliferation) and intracellular signaling mechanisms. METHODS Scratch wounds were created in monolayers of an immortalized human corneal epithelial cell (HCEC) line. The wounded monolayers were exposed to insulin...

The molecular circuitries controlling the process of skin wound healing have gained new significant insights in recent years. This knowledge is built on landmark studies on skin embryogenesis, maturation, and differentiation. Furthermore, the identification, characterization, and elucidation of the biological roles of adult skin epithelial stem cells and their influence in tissue homeostasis ha...

In epithelial cell movements, which occur during wound healing or embryonic morphogenesis, sheets of cells move together as a unit. Molecular mechanisms that regulate this sheet movement have been largely unknown, although cell locomotion or movement mechanisms for individual cells, such as for fibroblastic cells, have been extensively studied. Here, we show that, during wound healing, sheets o...

Ocular chemical injuries accounts for 11.5% to 22.1% of ocular injuries. burns are an ophthalmic emergency and requires immediate treatment. We report a series three cases injury secondary accidental exposure red balm used as local application headache. In our menthol, cajuput oil capsaicin might have contributed nerve damage. Nerve damage lead delayed epithelial healing. All patients improved ...