Exposure of Ehrlich ascites tumor cells to the polyanion dextran sulfate altered the permeability of the plasma membrane resulting in decreased glycolysis and respiration, enhanced permeability to Rb+ and adenosine 5'-triphosphate, and increased staining by erythrosin B. The inhibition of respiration was reversed by the addition of inorganic phosphate, whereas restoration of glycolysis required...

investigation. Single molecular TIRF microscopy was used to measure the translational diffusional coefficient of Alexa488 labeled monomeric PLB reconstituted into a supported lipid bilayer. The diffusional coefficient of monomeric PLB is 0.7 mm/s, which is consistent with its molecular weight. Timeresolved phosphorescence anisotropy of erythrosin iodoacetamide (ErIA) labeled SERCA in cardiac sa...

A procedure is described for visualization of cells that had only fetal hemoglobin apnormal and mutant adult hemoglobins in peared as clear ghosts. Analyses of cord human blood smears. After extraction of blood from newborn infants indicate that, blood smears with a concentrated poalthough most erythrocytes contain only Hb tassium phosphate buffer (2.76 M, pH 7.2), F and a few contain only Hb A...

Subnanosecond lifetime measurements using picosecond pulses from a mode locked Nd3+/glass laser together with conventional absorption and fluorescence yield methods have been used to study the photophysics of fluorescein and three of its halogenated derivatives (eosin, erythrosin, and rose bengal) in aqueous and simple alcoholic solvents. For each of the dye molecules absorption and fluorescenc...

OBJECTIVES The purpose of this study was to determine the plaque removal effectiveness of a new powered toothbrush and compare it to that of a manual brush. METHODS This examiner-blind, randomized study used a cross-over design. Sixty-two (62) qualifying subjects were randomly assigned either to the powered brush (Arm & Hammer Spinbrush™ Truly Radiant™ Clean & Fresh Battery-Powered Toothbrush...

The role of acid secretion in regulating short-term changes in growth rate and wall extensibility was investigated in emerging first leaves of intact, water-stressed maize (Zea mays L.) seedlings. A novel approach was used to measure leaf responses to injection of water or solutions containing potential regulators of growth. Both leaf elongation and wall extensibility, as measured with a whole-...

BACKGROUND Alzheimer's disease (AD) is the most common form of dementia. AD is a degenerative brain disorder that causes problems with memory, thinking and behavior. It has been suggested that aggregation of amyloid-beta peptide (Aβ) is closely linked to the development of AD pathology. In the search for safe, effective modulators, we evaluated the modulating capabilities of erythrosine B (ER),...

Dietary guidance to the public stresses foods or food groups as specific sources of minerals such as calcium in dairy products, iron in meats and legumes, and potassium in oranges and bananas. Dietary guidance also identifies foods that are low in some minerals (such as sodium, potassium, calcium, cooper, and iron) for patients with specific disease conditions. However, the levels of minerals a...

Cobra venom cytotoxins are basic three-fingered, amphipathic, non-enzymatic proteins that constitute a major fraction of cobra venom. While cytotoxins cause mitochondrial dysfunction in different cell types, the mechanisms by which cytotoxins bind to mitochondria remain unknown. We analyzed the abilities of CTI and CTII, S-type and P-type cytotoxins from Naja naja oxiana respectively, to associ...