same extent as M-( -)-propranolol (by 80%). Treatment of membranes with isoprenaline + GMP converts N from the closed GDP-bound state to an open Gpp(NH)presponsive form (Braun et af . , 1982). This form of N was found to be relatively insensitive to propranolol and also attenuated the filipin inhibition of Gpp(NH)p stimulation, but to a lesser extent. The reason for this is unclear, but may be ...

The preimplantation mouse blastocyst consists of two differentiated tissues, the trophectoderm (a structurally and functionally polarized epithelium) and the inner cell mass. The divergence of these two cell types can be traced back to a contact dependent polarization of the surface and cytoplasm at the 8-cell stage. Membrane/cytocortical organization during this preimplantation period has been...

background and objective: tuberculosis is still a major health problem, involving about 1/3 of the world´s population. diagnosis is difficult when we only use ziehl-neelson staining. many cases may be missed. a more rapid and sensitive diagnostic method is necessary. pcr may be helpful. the aim of this study was to compare pcr, zieh-neelsen staining and histopathologic findings in diagnosis of ...

Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in h...

Using filipin as a probe for the presence of membrane cholesterol, the evolution of cholesterol distribution in the apical plasma membrane was studied during estrogen-induced ciliogenesis in quail oviduct and compared with the distribution of intramembrane particles (IMPs). Ciliary growth is preceded by the first step of microvillus differentiation. Microvilli emerge in membrane domains rich in...

A mouse cell line (LM), which grows predominantly as spindle-shaped cells with numerous filopodia, was employed in this study. These filopodial projections appear to be important as sites of attachment to the substratum in LM cells. Morphologically the filopodia are slender projections from the cell body which usually attach to the substrate at their distal ends (filopodial footpads). Freeze-fr...

PURPOSE To determine the cholesterol composition of normal human Bruch's membrane and choroid as a function of age and retinal location. METHODS Human eyes with grossly normal maculas were preserved <4 hours after donor death. Cryosections of retina and choroid from the macula and temporal equator were stained with filipin to reveal esterified (EC) or unesterified (UC) cholesterol (n = 20, 17...