Real-time PCR and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis and compared to standard diagnostic procedures. For PCR, a LightCycler approach was chosen, implementing eubacterial and specific PCR assays for the most relevant bacteria. For FISH, a similar probe set containing eubacterial and specific probes was composed of p...

rapid and accurate identification of microorganisms have a significant impact on strategies and fish health management programs. hence, in this study a duplex pcr assay based on the 16s rrna gene for simultaneous detection of aeromonas hydrophila rticc 1032 and escherichia coli rticc 2325 from pure cultures, and challenged fish tissues was performed and their results were compared with the resu...

motile members of the genus Aeromonas, are among the most common and troublesome diseases of fish raised in ponds and recirculating systems. The widespread distribution of these bacteria in the aquatic environment and the stress induced by intensive culture practices predisposes fish to infections. Motile aeromonad infections have been recognized for many years and have been referred to by vari...

background and objectives: pseudomonas aeruginosa is one of the most important opportunistic pathogens in patients who suffer from immunodeficiency and burns. wound infections caused by p. aeruginosa can rapidly change to systemic and life threatening situation. the aim of this study was to apply florescent in situ hybridization (fish) method for rapid detection of p. aeruginosa in burrn patien...

In this study, the antimicrobial activity of three solvent extracts of consortium (mixed culture) of commercial available probiotic bacteria against eight fish pathogens using well diffusion and disc diffusion assay method. The probiotic bacteria inhibited almost all the fish pathogens tested. All tested strains were significantly inhibited at probability level (P<0.5).Zone of inhibition was hi...

We investigated the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria in domestic wastewater biofilms and autotrophic nitrifying biofilms by using microsensors and fluorescent in situ hybridization (FISH) performed with 16S rRNA-targeted oligonucleotide probes. The combination of these techniques made it possible to relate in situ microbial activity directly to th...