By sequencing tens of millions of TCRa transcripts from naive mouse CD8 T cells, Genolet et al (2012) show that the TCRa repertoire is at least as diverse as the TCRb repertoire. This overturns a long held view in the field that recombination of the Tcra locus occurs in a co-ordinate sequential bidirectional manner that relies on proximity of Va and Ja gene segments. The observation that rearra...

While most genes in the mammalian genome are transcribed from both parental chromosomes in cells where they are expressed, approximately 10% of genes are expressed monoallelically, so that any given cell will express either the paternal or maternal allele, but not both. The antigen receptor genes in B and T cells are well-studied examples of a gene family, which is expressed in a monoallelic ma...

background: acute lymphoblastic leukemia (all) is the most common form of childhood cancer leading to cancer-related death in children. most infants with all harbor recurring structural chromosomal rearrangements that are important initiating events in leukemogenesis but are insufficient to explain the biology and heterogeneity of the disease. mixed-lineage leukemia-rearrangement (mll-rearrange...

Thermal rearrangement of 7,7-dichloro-[a,c]-dibenzo-[4,1,0]-bicycloheptane (1) to 5,6-dichloro-5Hdibenzo-[a,c]-cycloheptene (2) was studied in the solid phase and in solvents with different polarities. Thefirst-order constants at various temperatures for the rearrangement process were evaluated from theabsorption time data. The activation parameters for this rearrangement were obtained from the...

A facility layout problem is concerned with determining the best position of departments, cells, or machines on the plant. An efficient layout contributes to the overall efficiency of operations. It’s been proved that, when system characteristics change, it can cause a significant increase in material handling cost. Consequently, the efficiency of the current layout decreases or is lost and it ...

B-lymphocyte development exhibits a characteristic order of immunoglobulin gene rearrangements. Previous work has led to the hypothesis that expression of the immunoglobulin mu heavy chain induces rearrangement activity at the kappa light-chain locus. To examine this issue in more detail, we isolated five matched pairs of mu- and endogenously rearranged mu+ cell lines from the Abelson murine le...