From the ocular connective tissues, relatively pure structural glycoproteins (SGP) are extractable by means of 8 m urea after trichloroacetic acid treatment. The SGP from corneal stroma (CSGP) and from sclera (SSGP) show considerable immunochemical similarities and are not related to SGP from anterior lens capsule (LSGP). An antiserum directed against the total connective tissue fraction of cor...

From the Department of Ophthalmology, the Oscar Johnson Institute, and the Division of Biostatistics, Washington University School of Medicine, St. Louis, Mo. This work was supported in part by NEI grants EY 00256 and EY 02687 (Dr. Moses) and NIGMS grant GM 28719 (Dr. Wette) from the National Institutes of Health, Bethesda, Md. Submitted for publication Aug. 17, 1981. Reprint requests: Robert A...

Secretory glycoproteins that fail to fold or assemble correctly are retained in the endoplasmic reticulum and eventually degraded. Recent evidence shows that trimming of their N-linked oligosaccharide chains plays a key role in targeting glycoproteins for destruction.

We report dynamic glycoprotein recognition of gels prepared by biomolecular imprinting using lectin and antibody molecules as ligands for tumor-specific marker glycoproteins. The glycoprotein-imprinted gels prepared with minute amounts of cross-linkers could dynamically recognize tumor-specific marker glycoproteins by lectin and antibody ligands and induce volume changes according to the glycop...

1. The concentrations of four serum glycoproteins, thyroxine-binding globulin, alpha 2-macroglobulin, alpha 1-antitrypsin and transferrin, as well as their reactivities with concanavalin A and lentil-lectin, were measured in patients with hepatocellular carcinoma or fulminant hepatic failure and in normal subjects. 2. Serum concentrations of thyroxine-binding globulin and alpha 1-antitrypsin we...

The mechanism of entry of hepatitis C virus (HCV) through interactions between the envelope glycoproteins and specific cell surface receptors remains unclear at this time. We have previously shown with the vesicular stomatitis virus (VSV)/HCV pseudotype model that the hypervariable region 1 of the HCV E2 envelope glycoprotein helps in binding with glycosaminoglycans present on the cell surface....

Cells adhere to the extracellular matrix through interaction with adhesive extracellular matrix glycoproteins, including fibronectin, laminins, vitronectin, thrombospondins, tenascins, entactins (or nidogens), nephronectin, fibrinogen, and others. Most adhesive glycoproteins bind cells through cell surface integrin receptors in conjunction with other cell surface receptors, such as dystroglycan...

Recycling of a number of glycoproteins along the site of action of mannosidase I (the distal endoplasmic reticulum/cis-Golgi) was followed in several different cell lines. Treatment of cells with 1-deoxymannojirimycin (dMM) produced high mannose oligosaccharides at positions otherwise occupied by complex-type oligosaccharides in these glycoproteins. Conversion of high-mannose-type oligosacchari...

The capacities of immature and mature rat brain myelin, bovine myelin and human myelin to be agglutinated by soya-bean agglutinin, Ricinus communis agglutinin, wheatgerm agglutinin, and Lotus tetragonolobus agglutinin were examined. The first two lectins, which are specific for galactose and N-acetylgalactosamine, strongly agglutinated immature and mature rat myelin, weakly agglutinated bovine ...