Recognition of the 3'-splice site is a key step in pre-mRNA splicing and accomplished by a dynamic complex comprising splicing factor 1 (SF1) and the U2 snRNP auxiliary factor 65-kDa subunit (U2AF65). Both proteins mediate protein-protein and protein-RNA interactions for cooperative RNA-binding during spliceosome assembly. Here, we report the solution structure of a novel helix-hairpin domain i...

INTRODUCTION The purpose of this study was to evaluate the long-term stability of quad-helix/crib treatment in subjects with dentoskeletal open bite. METHODS Twenty-eight subjects (11 boys, 17 girls; mean age, 8.2 ± 1.3 years) were treated consecutively with quad-helix/crib appliances. The patients were reevaluated at the end of active treatment with the quad-helix/crib (mean age, 9.7 ± 1.6 y...

We used UV resonance Raman (UVRR) spectroscopy exciting at ∼200 nm within the peptide bond π f π* transitions to selectively study the amide vibrations of peptide bonds during R-helix melting. The dependence of the amide frequencies on theirΨ Ramachandran angles and hydrogen bonding enables us, for the first time, to experimentally determine the temperature dependence of the peptide bond Ψ Rama...

This paper explores the superposition of ultrasonic vibration on vertical rotating augers in a variety granular media. Ultrasonic is known to facilitate direct penetration media, and it anticipated that any related reduction torque requirements system might improve augering performance. Experimental results suggest that, compared non-ultrasonic scenario, ultrasonically assisted significantly pr...

bioactive products from snail slime of “helix” specie have potential applications in preventing and/or treating several human diseases and in cancer diagnosis. however, the poor pharmacokinetics characteristics of these natural compounds limit their use. nanotechnology offers promising solutions for the enhanced formulation of these molecules through the synthesis of nanosized drug delivery sys...

Structures of three potassium channels of the six-trans-membrane (TM) helix type, a ligand-gated channel, and two voltage-gated channels were solved recently by x-ray crystallography (Long et al., 2005a,b, 2007; Clayton et al., 2008). In all three channel structures, the fourth TM segment (the voltage sensor in the voltage-gated channels) of each subunit adopts a 3 10-helical confor-mation over...