BMI1 is highly expressed in several malignant tumors, and its expression level is associated with tumor progression, proliferation, and prognosis. However, no published studies have examined the role of BMI1 in laryngeal squamous cell carcinoma (SCC). Expression of BMI1 in primary tumors was analyzed by immunofluorescence staining, real-time PCR, and Western blotting. BMI1 was knocked down, and...

17β-estradiol (E2) plays a key role in tumorigenesis by enhancing cell survivability and metastasis through its cytoplasmic receptors. Recently, a variant of estrogen receptor alpha, ERα36 has been implicated as a substantial mediator of E2's proliferative and antiapoptotic effects through rapid membrane-associated signaling, and cancers previously regarded as hormone-independent due to the abs...

Paclitaxel is a widely used chemotherapy drug for advanced laryngeal cancer patients. However, the fact that there are 20-40% of advanced laryngeal cancer patients do not response to paclitaxel makes it necessary to figure out potential biomarkers for paclitaxel sensitivity prediction. In this work, Hep2, a laryngeal cancer cell line, untreated or treated with lower dose of paclitaxel for 24 h,...

the aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2seal and ah plus, in two osteoblast-like cell lines, mg-63 and saos-2. using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. the extracts were exchanged with saos-2 or mg-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability t...

objective: bone marrow mesenchymal stem cells reside in bone marrow and support homing and differentiation of hematopoietic stem cells (1). this makes them an excellent instrument for regenerative treatment and co-culture with the hsc. materials and methods: in this study, k562 cell lines were treated with butyric acid(for erythroid differentiation), co-cultured with mesenchymal stem cells and ...

Three-dimensional (3D) cell cultivation is a powerful technique for monitoring and understanding diverse cellular mechanisms in developmental cancer and neuronal biology, tissue engineering, and drug development. 3D systems could relate better to in vivo models than two-dimensional (2D) cultures. Several factors, such as cell type, survival rate, proliferation rate, and gene and protein express...

The Huynh electronic parameter (HEP) is a modern alternative method to determine ligand donor strengths by 13C NMR spectroscopy of metal NHC complexes containing the interest. Using HEP, properties numerous classical Werner-type and organometallic ligands have been evaluated thus far. Moreover, it has extended HEP2, which provides means measure bidentate chelators. This Highlight Review covers ...