background: mentha species are widely used in traditional medicine mostly as anti-flatulence. nowadays, their usage as flavor and preservative in food, cosmetic and pharmaceutical industries has been developed. moreover, cytotoxic effects of some mentha species have been reported. objective: in this study, cytotoxic properties of mentha piperita, m. spicata, m. aquatica, m. crispa, m. pulegium ...

background and objective: in addition to the use of chemicals in food, pharmaceutical, cosmetic and hygiene industries, use of herbal and natural compounds have also increased. it seems natural ingredients compared with the chemical components show less side effects, such as mutagenicity and carcinogenesis however, their use can also be associated with side effects and toxicity. some significan...

The present study is an attempt to evaluate the standardization of crude mangosteen and γ – mangostin from Garcinia mangostana on MCF-7 (breast cancer cell line) and Hep2 (laryngeal cancer cell line). The study includes cell doubling time, ED50 analysis (growth inhibition), cell proliferation assay and assessment of cell cytotoxicity by dye exclusion assay. The result of the study showed that t...

BACKGROUND To investigate the expression and role of special AT-rich sequence-binding protein-2 (SATB2) in laryngeal squamous cell carcinoma (LSCC) tissue and cell line (HEp2), and to evaluate the clinical and prognostic significance of SATB2 protein in patients with LSCC. METHODS The expression of SATB2 was examined in LSCC tissue and HEp2 cells by Western-blotting, Real-time PCR and immunoh...

objective: as we are approaching the global eradication of wild poliovirus, laboratory surveillance of poliovirus by the gold standard cell culture method becomes increasingly important. as there is a lot of concern about accurate and sensitive detection of imported wild and vaccine derived polioviruses (vdpvs) in polio-free countries, in this study we assessed and compared the sensitivity of t...

BACKGROUND/AIM Surgery and chemotherapy treatments of human laryngeal squamous cell carcinoma (HLSCC) may fail due to metastasis, in which epithelial-mesenchymal transition (EMT) plays an important role. TRPP2, a nonselective cation channel, is expressed in various cell types and participates in many biological processes. Here, we show that TRPP2 enhanced metastasis by regulating EMT. METHODS...

The major obstacle of successful tumor treatment with carboplatin (CBP) is the development of drug resistance. In the present study, we found that following treatment with CBP the amount of platinum which enters the human laryngeal carcinoma (HEp2)-derived CBP-resistant (7T) cells is reduced relative to the parental HEp2. As a consequence, the formation of reactive oxidative species (ROS) is re...

Using biological medium, such as plant extract for the biosynthesis of nanoparticles is an ecofriendly and emerging scientific trend. On this back drop the present study focused on the biosynthesis of silver nanoparticles using Cissus quadrangularis and evaluation of its antimicrobial and anticancer potentials. The biosynthesized nanoparticle was characterized by UV-VIS spectroscopy, EDAX and T...

The combination of a novel topoisomerase II inhibitor, S16020, and ionizing radiation (IR) was investigated with the aim of assessing normal tissue tolerance using a mouse mucosal lip model and antitumor activity in a human carcinoma (HEP2) cell line. No increase of acute mucosal reactions was seen when combining S16020 with IR as compared with IR alone. Using clonogenic cell survival assay, a ...

It has been recently shown (Larkin, J. M., M. S. Brown, J. L. Goldstein, and R. G. W. Anderson, 1983, Cell, 33:273-285) that after a hypotonic shock followed by incubation in a K+-free medium, human fibroblasts arrest their coated pit formation and therefore arrest receptor-mediated endocytosis of low density lipoprotein. We have used this technique to study the endocytosis of transferrin, diph...