The present study characterized the ability of a bacterial cutinase to improve the wettability of raw cotton fabrics by specific hydrolysis of the cutin structure of the cuticle. The effect of cutinase was studied alone and in coreaction with pectin lyase. The changes in both the fabric and the reaction fluid were measured and compared to enzymatic hydrolysis with polygalacturonase, and to chem...

OBJECTIVE The objective of this study was to optimize ultrasonic-assisted enzymatic hydrolysis conditions, including enzyme-to-substrate (E/S) ratio, pH, and temperature, for producing porcine liver hydrolysates (PLHs) with the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity by using response surface methodology (RSM). METHODS The study used RSM to determine the ...

The effects of heat-induced denaturation and subsequent aggregation of whey protein isolate (WPI) solutions on the rate of enzymatic hydrolysis was investigated. Both heated (60 °C, 15 min; 65 °C, 5 and 15 min; 70 °C, 5 and 15 min, 75 °C, 5 and 15 min; 80 °C, 10 min) and unheated WPI solutions (100 g L(-1) protein) were incubated with a commercial proteolytic enzyme preparation, Corolase PP, un...

Vine shoots are agricultural residues that can be used as a raw material in agro-biorefineries, which their main constituents individually converted into valuable bioproducts. The treatment of vine uncatalyzed media containing water and 1-butanol enabled the single-stage separation major different phases: aqueous phase from treatments contained hemicellulose-derived products (mainly form oligos...

The effects of limited enzymatic hydrolysis with trypsin on the physicochemical and functional properties of buckwheat protein isolate (BPI) were investigated. The protein constituents, free sulfhydryl (SH) contents and surface hydrophobicity (Ho), as well as some selected functional properties, such as protein solubility (PS), emulsifying (including emulsifying activity index, EAI and emulsion...

BACKGROUND Casein is the main proteinaceous component of milk and has made us interest due to its wide applications in the food, drug, and cosmetic industries as well as to its importance as an investigation material for elucidating essential questions regarding the protein chemistry. Enzymatic hydrolysis is an important method commonly used in the modification of protein structure in order to ...

Protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in Saccharomyces cerevisiae and Kluyveromyces marxianus. Crude protein content of dried yeast cells were 53.22% and 45.6% for S.cerevisiae and K.marxianus, respectively. After 96 hrs of...

The hydrolysis of sodium borohydride as a source of hydrogen gas is studied at different mole ratios of H+ to NaBH4. The sulfonated porous carbon (SPC) is used as a source of hydrogen ion and catalyst. It is found that the sulfonated porous carbon exhibits high activity for the hydrolysis of NaBH4 to generate hydrogen gas at room temperature in comparison to Amberlyst-15 and Nafion-NR50. The ki...

The present paper addresses two crucial features in the industrial development of fructose production by enzymatic hydrolysis of inulin: the use of immobilized biocatalyst in the hydrolysis of crude extracts of chicory roots and the evaluation of the effect of degree of polymerization of inulin on the overall reaction rate. The immobilized biocatalyst consisted of inulinase covalently bound to ...