bstract—Although multiple effects of GABAB receptor actiation on synaptic transmission in the striatum have been escribed, the precise locations of the receptors mediating hese effects have not been determined. To address this ssue, we carried out pre-embedding immunogold electron icroscopy in the rat using antibodies against the GABAB eceptor subunits, GABAB1 and GABAB2. In addition, to inesti...

Legionella pneumophila is a facultative intracellular pathogen that parasitizes host mononuclear phagocytes. Cell-mediated immunity is pivotal to host defense against L. pneumophila, and the infected host cell may play a central role in processing and presenting parasite antigens to lymphocytes mediating cell-mediated immune response. However, in the case of L. pneumophila and intracellular par...

P/Q-type voltage-dependent calcium channels play key roles in transmitter release, integration of dendritic signals, generation of dendritic spikes, and gene expression. High intracellular calcium concentration transient produced by these channels is restricted to tens to hundreds of nanometers from the channels. Therefore, precise localization of these channels along the plasma membrane was lo...

The Charcot-Leyden crystal (CLC) protein is a unique constituent of eosinophils and basophils. This protein forms the hexagonal bipyramidal crystals observed in tissues at sites of eosinophil accumulations, possesses lysophospholipase activity (lysolecithin acylhydrolase E.C.3.1.1.5), and comprises an estimated 7% to 10% of total eosinophil protein. The ultrastructural localization of CLC prote...

On the basis of autoradiographic studies, it used to be accepted that rDNA transcription takes place in the dense fibrillar component of the nucleolus (reviewed by Goessens, 1984; Fakan, 1986). Over the past few years, however, data based on immunolocating procedures at the electron microscope level have strongly suggested that rDNA transcription occurs in the fibrillar centres (Scheer and Bena...

We examined the distribution of the non-clathrin-coated vesicle-associated coat protein beta-COP in rat exocrine pancreatic cells by immunogold cytochemistry. Labeling for beta-COP was found in the Golgi region (48%) where it was associated with vesicles and buds of approximately 50 nm, showing a characteristic approximately 10-nm-thick coat. The other half of the label was present in the cytop...