in order to determine the most effective hormones on the in vitro oocyte maturation (germinal vesicle breakdown - gvbd) in a hybrid sturgeon bester, ovarian follicles were incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin preparations and forskolin. occurrence of gvbd and 17,20β-dihydroxy-4-pregnen-3 -one (dhp) production were followed during an expe...

the aim of the present study was to investigate the effect of follicle stimulating hormone (fsh) on survival, activation and growth of ovine primordial follicles using histological studies. pieces of ovine ovarian cortex were obtained and cultured for 6 days in minimum essential medium supplemented with or without fsh (50 ng/ml). these fragments were then process to be used for histology compar...

plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. photoautotrophic and photomixotrophic systems could diminish these problems. the purpose of this study was to increase the chlorophyll...

Background: Cystic echinococcosis (CE), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. It enhances both humoral and cellular (Th1 and Th2) responses in infected host. Different antigens of the worm may favor the Th1 or Th2 immune responses in CE patients. Objective: To evaluate the humoral and cellular immune responses of Balb/c mice agai...

Background: Surface properties of a biomaterial could be critical in determining biomaterial’s biocompatibility due to the fact that the first interactions between the biological environment and artificial materials are most likely occurred at material’s surface. In this study, the surface properties of a new nanocomposite (NC) polymeric material were modified by combining plasma treatment and...

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

Weak microwave radiation (WMR) in our environment has raised health concerns in the public. Among those, communication frequencies are more than ever becoming widespread and their effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments shall preferentially use primary cells. In this study we compared non-modulated (CW) and modulated WMR exposure o...