organotypic spinal cord slices from neonatal mammals could be a powerful model for evaluation of cell survival but also cell death mechanisms. the aim of this study was to establish an in vitro model for investigating cell survival and mechanism involved in cell death in neonatal spinal cord slices. the spinal cord was sliced and incubated into culture medium. the mtt assay was carried out to a...

A highly potent inhibitor of calmodulin-sensitive phosphodiesterase (PDE) activity was isolated from the culture broth of an unidentified fungal isolate, SCF-125. A chemically defined medium was developed for production of this compound. The PDE inhibitor was isolated from the fermentation filtrate by adsorption on a macro-reticular resin and further purified by gel filtration chromatography an...

In this paper, the effective parameters on the ductility demand of the seismically base isolated structure are investigated, and then a relation between the strength reduction factor and the target ductility is presented. The investigation has been conducted by modelling the base isolated structure as a two degree of freedom model in the OpenSees software, and the possibility of yielding in the...

the isolated fungi species of different kinds from chromium contaminated soil sites located in nagalkeni, chennai were used for reducing chromium(vi) in a tannery industry wastewater of nagalkeni, chennai.  the experiments were conducted to know biosorption potential of isolated fungi species for removing chromium(vi) in a tannery industry wastewater against the different ph, fungi biomass and ...

AIMS To investigate the outcomes of Rho-kinase inhibition in the electrophysiological ex vivo model of the isolated perfused vertebrate retina under hypoxia. METHODS Bovine retinas were perfused with an oxygen saturated nutrient solution with or without the Rho-kinase inhibitor H-1152P. The retinas were stimulated repeatedly until stable amplitudes were reached and the electroretinogram was r...

To produce an active metabolite of Chlordiazepoxide by fungal biotransformation in easy and economic way also to develop microbial models for drug metabolism studies. is metabolized the liver CYP3A4 forms major N-desmethyl chlordiazepoxide. The focus study was explore ability six distinct fungi biotransform its metabolites. Induction, Inhibition kinetic studies were conducted find out type capa...

Background and Objective: In temporal lobe epilepsy (TLE), recurrent seizures accompany with cognitive deficit. In some patients, the current medications cannot provide satisfactory control of seizures, therefore new drugs that act through different mechanisms are required. In the present study, the useful effect of dipeptidyl peptidase-4 inhibitor was evaluated in experimental model of tempora...