The maturation and packaging of bacteriophage lambda DNA are under the control of the multifunctional viral terminase enzyme, which is composed of the protein products of Nu1 and A, the two most leftward genes of the phage chromosome. Terminase binds selectively to the cohesive end site (cos) of multimeric replicating lambda DNA and introduces staggered nicks to regenerate the 12-base single-st...

A 70-year-old man presented with a 6-year history of oedema of both lower extremities. Ultrasonic examination showed bilateral anterior tibial artery occlusion due to thrombosis, moderate bilateral pleural effusions and ascites. He had severe pancytopenia, chronic renal dysfunction (serum creatinine 223 8 lmol/l and more than 1 g/24 h of urinary protein) and, on ultrasound examination, mildly e...

The fluorescence of tryptophan is used as a signal to monitor the unfolding of proteins, in particular the intensity of fluorescence and the wavelength of its maximum lambda(max). The law of the signal is linear with respect to the concentrations of the reactants for the intensity but not for lambda(max). Consequently, the stability of a protein and its variation upon mutation cannot be deduced...

Beta-sulfoquinovosyldiacylglycerol (betaSQDG) is a synthetic sulfoglycolipid that shows inhibitory activity of DNA polymerase lambda (pol lambda). Here we identified a betaSQDG binding region within murine pol lambda (Mmpol lambda) using T7 phage display technology. We compared the binding intensity of betaSQDG with recombinant phages (phages lambda1-6) that displayed different segments of Mmpo...

The metallointercalator Lambda-1-Rh(MGP)2phi5+ binds tightly and specifically to the site 5'-CATATG-3' in the major groove of double helical DNA by a combination of direct readout and shape selection. To examine competitive interactions between this small metal complex and a DNA-binding transcription factor, the preferred binding site for Lambda-1-Rh(MGP)2phi5+ was engineered into the AP-1 reco...

The correct replication and repair of DNA is critical for a cell's survival. Here, we investigate the fidelity of mammalian DNA polymerase lambda (pol lambda) utilizing dynamics simulation of the enzyme bound to incorrect incoming nucleotides including A:C, A:G, A(syn):G, A:A, A(syn):A, and T:G, all of which exhibit differing incorporation rates for pol lambda as compared to A:T bound to pol la...

Recall that a continuous function $fcolon Xto Y$ between Tychonoff spaces is proper if and only if the Stone extension $f^{beta}colon beta Xtobeta Y$ takes remainder to remainder, in the sense that $f^{beta}[beta X-X]subseteq beta Y-Y$. We introduce the notion of ``taking remainder to remainder" to frames, and, using it, we define a frame homomorphism $hcolon Lto M$ to be $beta$-proper, $lambd...