Argonaute (Ago) proteins and microRNAs (miRNAs) are central components in RNA interference, which is a key cellular mechanism for sequence-specific gene silencing. Despite intensive studies, molecular mechanisms of how Ago recognizes miRNA remain largely elusive. In this study, we propose a two-step mechanism for this molecular recognition: selective binding followed by structural re-arrangemen...

Markov state models (MSMs) have become a popular approach for investigating the conformational dynamics of proteins and other biomolecules. MSMs are typically built from numerous molecular dynamics simulations by dividing the sampled configurations into a large number of microstates based on geometric criteria. The resulting microstate model can then be coarse-grained into a more understandable...

A similar error occurs in the figure legend for Supplementary Fig. 16. The figure legend should read: ‘Supplementary Fig. 16: Distribution of simulated trajectory lengths. For the (a) apo and (b) holo CaM systems, 12,184 and 9,995 trajectories were simulated for a total time of 256 and 455 ms, respectively. Trajectories shorter than the lag time at which the MSMs were built were eliminated from...

Introduction: It is believed that Locus Coeruleus (LC) influences the sensory information processing. However, its role in cortical surround inhibitory mechanism is not understood. In this experiment, using controlled mechanical displacement of whiskers we investigated the effect of phasic electrical stimulation of LC on response of layer V barrel cortical neurons in anesthetized rat. Methods: ...

introduction: noradrenergic cells in lc participate in the process of cortical activation and behavioral arousal. the evidence suggests that locus ceoruleus (lc) plays an important role in the sleep-wake cycle. the aim of this study was stereological estimation of cavity caused by lesion and assessment of sleep stages after bilateral lesion of the lc. materials and methods: male wistar rats wei...

Background and Purpose:  Due to the important role of imaging in the diagnosis and treatment plan in orthodontics, CBCT (Cone Beam Computed Tomography) images because of their three-dimensional nature, can minimize the disadvantages of two-dimensional images such as magnification, distortion, or superimposition. The purpose of this study was to evaluate the accuracy of conventional cephalograms...

background: to glean insights into the relationship among hepatitis b virus (hbv) genotype/subgenotypes, a1762t/g1764a mutations and advanced liver disease such as liver cirrhosis (lc) and hepatocellular carcinoma (hcc) in southeast china. methods: a case-control study was performed, consisting of chronic hepatitis b (chb) patients (n=160), lc patients (n=150), and hcc patients (n=156). fluores...

introduction: it has been shown that orexin peptides have a role in opioid withdrawal behaviors. orexin-expressing neurons that are present in the hypothalamic nuclei send dense projections to the locus coeruleus (lc). withdrawal syndrome is temporally associated with hyperactivity of lc neurons. however, lc neurons do not show withdrawalinduced hyperactivity in the brain slices from morphine-d...

Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity. Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from patients with and without otomastoiditis. Methods The bone dust created during mastoidectomy, as well as the mastoid tissue, was ana...

Marginal structural models (MSMs) and inverse probability weighting can be used to estimate risk in a cohort of active workers if there is a time-varying confounder (e.g., health status) affected by prior exposure-a feature of the healthy worker survivor effect. We applied Cox MSMs in a study of incident ischemic heart disease and exposure to particulate matter with aerodynamic diameter of 2.5 ...