Journal of Biotechnology & Bioresearch Cistus ladanifer L. Tissue Culture from Leaf and Stem Explants David Franco Frazão1,2, Celina Barroca2,3, Amélia M Silva1,4, Fernanda Delgado2,3,5 José Carlos Gonçalves2,3,5* 1Center for Research Technology Agro-Environmental Biological Sciences (CITABUTAD), University Trás-os-Montes e Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portugal 2Plant Cente...

Nitraria schoberi (Ghar-e-Daghin in Persian) from Zygophyllaceae (Nitrariaceae) family is considered as a wonderful exquisite plant in terms of its economical, medicinal, animal feed, significant values and also its unique characteristics of being tolerant to the soil salinity, drought and erosion. On the other hand, this plant is challenging with severe problems such as seed dormancy, plant gr...

From stages 35 to 40 in the chicken embryo, ciliary ganglion neurons undergo a developmental change which is detected in our assay system as a marked decline in the ability to extend neurites when placed in culture. This developmental loss is observed when the neurons are placed in culture as single, dissociated cells or as undissociated ganglion explants. The loss of the ability to extend neur...

Abstract Taxol is an anticancer drug that widely used in cancer treatment worldwide. Since the source of a plant, increase its production one concerns researchers. One way to use stimulants for secondary metabolite production. Therefore, this study, methyl jasmonate was as bioelicitor, and effect on evaluated. For purpose, Taxus baccata elicited 48 72 hours with concentrations at 0, 100, 250, 5...

An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric ac...

It is of interest to apply plant tissue culture to generate plants resistant to toxic effects of cadmium (Cd) on plant growth. Callus cultures were initiated from leaf explants of micropropagated potato plantlets (Solanum tuberosum L., cv. Iwa) for in vitro selection comprising 18 different Cd treatments varying in Cd exposure timing and duration. Plantlets regenerated from two different lines ...

In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10-15 year old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) suppleme...

Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentia...