Ribosomes were isolated and ribosomal RNA (rRNA) was prepared from leukemic cells derived from the venous blood of patients with acute myeloblastic leukemia, acute undifferentiated leukemia, subacute myelocytic leukemia, chronic lymphocytic leukemia, leukemic lymphosarcoma, leukemic reticuloendotheliosis, a cultured Burkitt lymphoma cell line, and phytohemagglutinin-stimulated normal lymphocyte...

Two receptors for tumor necrosis factor (TNF) with different molecular weight (75-Kd and 55-Kd) and binding affinity have been recently discovered. To investigate the distribution and the functional role of these receptors on leukemic B cells from hairy cell leukemia (HCL) and B-cell chronic lymphocytic leukemia (B-CLL) patients, we evaluated: (1) the cytofluorimetric pattern of uncultured and ...

We investigated the surface expression of leukocyte differentiation antigens and the Ig heavy-chain variable region (VH) gene family use in leukemic cells from 26 Japanese patients with chronic B-cell leukemias with special reference to CD5 antigen expression. CD5 was expressed on leukemic cells in 21 of 26 cases (CD5+) but not in 5 cases (CD5-). Myelomonocytic marker, CD13 antigen was expresse...

An experimental animal model of meningeal leukemia was developed in the nude rat, rnu/rnu, using the human-derived acute lymphoblastic leukemia cell line HPB-ALL. Anesthetized rats were placed in a modified stereotaxic frame and then injected intrathecally, at the level of the cisterna magna, with human leukemic cells. Cerebrospinal fluid and tissue samples from brain, spinal cord, heart, liver...

LL PRESENT FORMS OF THERAPY for acute myelocytic leukemia (AML) injure or destroy normal as well as leukemic cells. The action of anti-metabolic chemotherapeutic drugs is largely directed at disrupting flucleic acid synthesis and, thus, replication of cells. The alkylating agents and whole body radiation are effective in reducing cell production by slowing the generative cycle and directly kill...

Human acute leukemic blood cells contain ribonucleic acid (RNA) and reverse transcriptase which are specifically related to analogous components in type-C viruses isolated from a lymphomosarcoma and from a fibrosarcoma which developed spontaneously in two different subhuman primate species. By appropriately using molecular probes specific for these viral components, it may be possible to detect...

Ribosomes were isolated and ribosomal RNA (rRNA) was prepared from leukemic cells derived from the venous blood of patients with acute myeloblastic leukemia, acute undifferentiated leukemia, subacute myelocytic leukemia, chronic lymphocytic leukemia, leukemic lymphosarcoma, leukemic reticuloendotheliosis, a cultured Burkitt lymphoma cell line, and phytohemagglutinin-stimulated normal lymphocyte...

bone marrow specimens from leukemic patients were studied in a 5 year period beginning in 1982 in children's medical center of teheran,a teaching and referral center for pediatric medicine. the slides were stained by giemsa-wright stains, read by at least two persons experienced in bone marrow pathology and classified according to f.a.b. recommendations. peroxidase or sudan black-b and p.a...

The effect of Adriamycin and ricin, singly and together, on the survival of L1210 leukemic cells in mice has been assessed by spleen colony and end-point dilution assays. Adriamycin, as well as ricin, had a stronger effect on leukemic than on normal bone marrow cells. Rapidly proliferating normal cells were as sensitive to Adriamycin as were the leukemic bone marrow cells. In contrast, the pref...

CD19-directed immunotherapies have revolutionized the treatment of advanced B-ALL. Despite initial impressive rates complete remission (CR) many patients ultimately relapse. The fact that B-ALL successfully treated with T-cells eventually relapse, coupled early onset CD22 expression during B-cell development suggest pre-existing CD34+CD22+CD19- (pre)-leukemic cells could represent an “early pro...