BACKGROUND Two mammary lymphatic cannulation models in sheep have been described with minimal use in the past 50 years. The purpose of this study was to investigate a new surgical technique to allow long term monitoring of mammary lymph flow and composition from the mammary glands, with rapid ewe recovery and minimal complications post-surgery. RESULTS We developed a modified methodology for ...

OBJECTIVE To evaluate the effects of Chinese herbal medicine for warming kidney on proliferation of normal human mammary epithelial cells in primary culture. METHODS The normal human mammary epithelial cells were dissociated by digestion with collagenase type I. The morphological identification of normal human mammary epithelial cells in primary culture was determined under inverted phase con...

Background/Aim: Our understanding of cancer risk from neutron exposure is limited. We aimed to reveal the characteristics mammary carcinomas induced by neutrons. Materials and Methods: Mammary obtained female Sprague-Dawley rats irradiated at 7 weeks age with 0.97 Gy neutrons or 4 ?-rays non-irradiated were classified into luminal non-luminal subtypes immunohistochemistry. Their mutational land...

The development of many epithelial organs depends on a series of sequential and reciprocal interactions between epithelial cells and adjacent mesenchymal or stromal cells (Thesleff et al., 1995; Birchmeier and Birchmeier, 1993). The mammary gland is an example of an organ where these epithelialmesenchymal interactions play a critical role, especially during embryonic development (Sakakura, 1987...

Studies in rodent models of breast cancer show that exposures to dietary/hormonal factors during the in utero and pubertal periods, when the mammary gland undergoes extensive modeling and re-modeling, alter susceptibility to carcinogen-induced mammary tumors. Similar findings have been described in humans: for example, high birthweight increases later risk of developing breast cancer, and dieta...

BACKGROUND The objective of this study was to establish the buffalo mammary epithelial cell line (BuMEC) and characterize its mammary specific functions. METHODOLOGY Buffalo mammary tissue collected from the slaughter house was processed enzymatically to obtain a heterogenous population of cells containing both epithelial and fibroblasts cells. Epithelial cells were purified by selective tryp...

This investigation evaluated mammary cell loss and replacement during lactation and the impact of administration of bST on these processes. During lactation, a gradual decrease in number of mammary epithelial cells within the mammary glands occurs and largely accounts for the decline in milk production with advancing lactation. This decrease is not appreciably impacted by the loss of viable epi...

The development of the mammary gland is unique: the final stages of development occur postnatally at puberty under the influence of hormonal cues. Furthermore, during the life of the female, the mammary gland can undergo many rounds of expansion and proliferation. The mammary gland thus provides an excellent model for studying the ‘stem/progenitor’ cells that allow this repeated expansion and r...

Breast cancer manifests itself in the mammary epithelium, yet there is a growing recognition that mammary stromal cells also play an important role in tumorigenesis. During its developmental cycle, the mammary gland displays many of the properties associated with breast cancer, and many of the stromal factors necessary for mammary development also promote or protect against breast cancer. Here ...

Spontaneously occurred canine mammary tumours (55) were treated with chemotherapy (Methotrexate), combination chemotherapy with COX-2 inhibitor (Meloxicam) and by surgical therapy. In chemotherapy groups Hb and TEC values reduced significantly (P<0.05) from the base line value at 3rd week of therapy. Histologically, benign and malignant mixed mammary tumours were predominant. Methotrexate and C...