Thioridazine (TZ) is used mainly in the treatment of schizophrenia. However, hepatotoxicity as a life-threatening adverse effect is associated with its clinical use. In this context, we examined the cytotoxic mechanisms of TZ on freshly isolated rat hepatocytes to better understanding of the pathogenesis of TZ-induced hepatotoxicity. Hepatocytes were prepared by the method of collagenase enzyme...

Mitochondria were simultaneously isolated from striatum and cortex of adult rats and compared in functional assays for their sensitivity to calcium activation of the permeability transition. Striatal mitochondria showed an increased dose-dependent sensitivity to Ca2+ compared with cortical mitochondria, as measured by mitochondrial depolarization, swelling, Ca2+ uptake, reactive oxygen species ...

The specificity of Ca2+ signals is conferred in part by limiting changes in cytosolic Ca2+ to subcellular domains. Mitochondria play a major role in regulating Ca2+ in neurons and may participate in its spatial localization. We examined the effects of changes in the distribution of mitochondria on NMDA-induced Ca2+ increases. Hippocampal cultures were treated with the microtubule-destabilizing ...

Methods Ascaridole was synthesized from alpha-terpinene and characterized by NMR and IR spectroscopy. The toxic effects of these compounds on macrophages from BALB/c mice and on the bioenergetics of submitochondrial particles from bovine heart (SMP) and rat liver mitochondria (RLM) were studied. Toxic radical intermediates arising from the endoperoxide ascaridole were characterized by ESR spect...

Impaired dynamics and function of mitochondria caused by mtDNA toxicity 1 leads to heart failure 2 3 Knut H. Lauritzen*, Liv Kleppa, Jan Magnus Aronsen, Lars Eide, Harald Carlsen, 4 Øyvind P. Haugen, Ivar Sjaastad, Arne Klungland, Lene Juel Rasmussen, Håvard 5 Attramadal, Jon Storm-Mathisen, and Linda H. Bergersen* 6 7 a Brain and Muscle Energy Group, Department of Oral Biology, University of O...

Maternal smoking has been recognized as a common cause of low birth weight, preterm birth and the decrease of gestational age period. Unfortunately, there is an increasing interest within public especially woman in Iran in the tobacco products consumption. On the other hand, the deleterious effect of maternal smoking on human fetus in pregnancy period especially in the first trimester encourage...

Alzheimer's disease (AD) brain reveals high rates of oxygen consumption and oxidative stress, altered antioxidant defences, increased oxidized polyunsaturated fatty acids, and elevated transition metal ions. Mitochondrial dysfunction in AD is perhaps relevant to these observations, as such may contribute to neurodegenerative cell death through the formation of reactive oxygen species (ROS) and ...

Alzheimer’s disease (AD) brain reveals high rates of oxygen consumption and oxidative stress, altered antioxidant defences, increased oxidized polyunsaturated fatty acids, and elevated transition metal ions. Mitochondrial dysfunction in AD is perhaps relevant to these observations, as such may contribute to neurodegenerative cell death through the formation of reactive oxygen species (ROS) and ...