We were recently asked to examine a large group of isolates of C. perfringens obtained from piglets with classical hemorrhagic, necrotic enteritis to confirm that they were type C. In fact, 95% were type A, and considering that true type-C cases yield pure cultures of type C, it seems unlikely that these were “normal flora” type-A strains picked inadvertently from a background of disease-causin...

We compared ileal Clostridium perfringens quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis i...

A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of Institute Veterinary Medicine Serbia for determining potential cause death. Necropsy revealed massive hemorrhages in subcutaneous and intermuscular tissue on papillary muscle. Petechiae ecchymoses were found omentum, mesentery adipose heart, kidney costal pleura. Haemorrhagic-necrotic enteritis duodenum jejun...