Serial analysis of gene expression (SAGE) is a widely used and powerful technique to characterize and compare transcriptomes. Although several modifications have been proposed to the initial protocol with the aim of reducing the amount of starting material, unless additional PCR steps are added, the technique is still limited by the need for at least 1 micro g of total RNA. As extra PCR amplifi...

background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cul...

The transfer of extracellular RNA is emerging as an important mechanism for inter-cellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes within recipient cells. The study of extracellular RNA requires sensitive methods for the detect...

conclusions this study showed that this novel one-step rrt-pcr assay is a rapid, reliable, repeatable, specific, sensitive, and simple tool for the detection of the cchf virus. results from a total of 75 suspected serum samples, 42 were confirmed to be positive for cchf virus, with no false-positives detected by the sequencing results. after 40 amplification cycles, the melting curve analysis r...

The virus-associated (VA) RNAI gene in human adenovirus 2 DNA has been shown by Wu (Wu, G. J. (1978) Proc. Natl. Acad. Sci. U. S. A. 75, 2175--2179) to be transcribed by RNA polymerase III in a human KB cell-free extract. In the present report we have examined the fidelity of transcription of adenovirus 2 DNA and Xenopus oocyte 5 S DNA templates by RNA polymerase III in extracts derived from cu...

The use of molecular techniques such as quantitative RT-PCR depends on the quality of cellular RNA. In particular, RNA extraction from endoscopic biopsies is difficult with respect to yield, and especially integrity. Endoscopic biopsies taken from the gastric antrum, corpus and duodenum were subjected to various RNA extraction protocols, and the RNA was used for quantitative RT-PCR. The subsequ...

background bone marrow and adipose tissue are two main sources of mesenchymal stem cells (mscs). some of studies suggest that there are some differences in gene expression profile of mscs-derived from various tissues. to investigate gene expression profile by qrt-pcr, an appropriate reference gene with stable expression level should be chosen for normalizing data.  this study was designed to ev...

micronemes are secretory organelles of the invasive stages of apicomplexan parasites and contain proteins that are important for parasite motility and host cell invasion. even though coccidiosis is a complex disease that can be caused by any combination of mainly seven species, most of the molecular researches concerning characterization of host and parasite genes and proteins have been limited...