نتایج جستجو برای: pcr reaction is almost twice compared with rt
تعداد نتایج: 11403121 فیلتر نتایج به سال:
Background: In the current COVID-19 pandemic, there is a rising need for a rapid and reliable diagnostic tool. We hypothesized that chest computed tomography (CT) can be a potential alternative for reverse transcription-polymerase chain reaction (RT-PCR). The aim of this study was to compare the diagnostic value of chest CT and RT-PCR in Iranian patients with suspected COVID-19. Methods: In a...
Mycobacterium tuberculosis is the bacterium that causes tuberculosis (TB), a leading cause of death from infectious disease worldwide. Rapid diagnosis of resistant strains is important for the control of TB. Real-time polymerase chain reaction (RT-PCR) assays may detect all of the mutations that occur in the M. tuberculosis 81-bp core region of the rpoB gene, which is responsible for resistance...
in this study, a simple, rapid and selective method was developed for the determination of dexamethasone. the proposed method is based on inhibitory effect of dexamethasone on the oxidation of orange-g by bromate in sulfuric acid media. the reaction was followed spectrophotometrically at 478.5 nm (?max). under optimum experimental conditions, (72.6 ?mol l-1 of orange-g, 0.76 mol l-1 of h2so4, 0...
Sensitive detection of water- and foodborne enteric viruses is extremely relevant, especially due to the low concentrations in which they are found. Accurate and sensitive detection of Norovirus, the primary responsible for water- and foodborne outbreaks, is of particular importance. Quantification of Norovirus is commonly performed by quantitative RT-PCR (RT-qPCR). In recent years a new platfo...
A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this eDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologu...
Powdery mildew (PM - Eryshpe necator) is one of the most important diseases threatening world viticulture. This study determined that gene expression levels Myc2, WRKY1, and DHN1a in Boğazkere (V. vinifera L. PM susceptible), Cabernet Sauvignon Kishmish Vatkana tolerant), Regent tolerant) cultivars after PM-Methyl jasmonate (MeJa) treatments by real-time polymerase chain reaction (RT-PCR). The ...
after test and review results and analysis of farm data, the following results were obtained. the review examined farms was done and the results obtained in this study was to determine the effectiveness of vaccines hatchpak avinew (frozen) and hatchpak ib h120 with spray in broilers a day in factories incubation and at least,they can be as vaccines avinew and bioral h120 ability to create saf...
Duck spleen necrosis disease (DSND) caused by Novel Reovirus (NDRV), is an emerging infectious that causes severely threaten to duck industry. Currently, the popular conventional RT-PCR technique for detecting NDRV time consuming. So, it essential develop a rapid and accurate molecular diagnosis techniques of pathogen purpose effective control disease. In our study, simple, reliable detection m...
we describe here a suitable approach for the synthesis of n-unsubstituted monocyclic b-lactams under mild reaction conditions by the annelation of imines with substituted acetylchlorides. in this method the reaily available phtalimidoacetyl chloride were allowed to react with - dibenzylideneiminotoluene (hydrobenzamide) in the presence of an equimolar amount of triethylamine in refluxing toluen...
To assess the performance of second reading chest compute tomography (CT) examinations by expert radiologists in patients with discordance between reverse transcription real-time fluorescence polymerase chain reaction (RT-PCR) test for COVID-19 viral pneumonia and CT report. Three hundred seventy-eight were included this retrospective study (121 women 257 men; 71 years median age, a range 29–93...
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