نتایج جستجو برای: pcr using homologous primers
تعداد نتایج: 3564282 فیلتر نتایج به سال:
Background and purpose: The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines are altered in Helicobacter pylori (H. pylori) infected gastric mucosa. Matrix metalloproteinases (MMP) are a family of zinc-dependent homologous enzymes digesting most of the components of the extracellular matrix and basement membrane and are involved in remodeling and functioning o...
Using several consensus sequences for 106 amino acid residue α-spectrin repeat segment as well as ours developed by using a genetic semihomology algorithm as probes we searched plant and S. cerevisiae databases by using BLAST programme in order to find proteins similar to spectrin. Among many sequences showing rather high level of homology in very short (few amino acid residues) segments we fou...
The polymerase chain reaction (PCR) is an indispensable DNA amplification technique that has been exploited in numerous areas, including molecular diagnostics. One major drawback of PCR is the competing amplification of undesired off-target products, which primarily occurs at ambient temperatures prior to PCR cycling (1). Hot Start PCR techniques aim to block the extension of primers in nonspec...
A novel real-time quantitative polymerase chain reaction (PCR) method using an attached universal template (UT) probe is described. The UT is an approximately 20 base attachment to the 5' end of a PCR primer, and it can hybridize with a complementary TaqMan probe. One of the advantages of this method is that different target DNA sequences can be detected employing the same UT probe, which subst...
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