the objective of this study was to determine the prevalence and distribution of chlamydia trachomatisgenotypes in shiraz, iran. two hundred twelve cervical swab samples were collected from women attendingshahid motahari polyclinic in shiraz, iran. the endocervical specimens were screened for c. trachomatis byplasmid pcr. genotyping was performed in c. trachomatis-positive samples by nested pcr ...

Abstract Background Patients with sterile pyuria may be infected sexually transmitted diseases or have renal tuberculosis. This study investigated the possibility of detecting and Mycobacterial tuberculosis in samples polymerase chain reaction. Methods Forty-four day-3 negative urine culture were for presence Results Among 44 samples, 61.4% positive by reaction (PCR) bacterial DNA (either Mycob...

The diagnostic performance of a PCR test (Roche Cobas Amplicor CT/NG Test) and that of a ligase chain reaction (LCR) test (Abbott LCx Chlamydia trachomatis assay) were compared by using endocervical and urethral swab specimen culture as a reference test. First-void urine (FVU) and endocervical and urethral swab specimens were collected from 1,015 unselected patients attending a sexually transmi...

Methods A total of 22 isolates, K. pneumoniae (n=20), Escherichia coli (n=1) and Enterobacter aerogenes (n=1), were collected from a university hospital, in Lisbon, Portugal. MIC were determined by Etest and interpreted according to Clinical and Laboratory Standards Institute guidelines. PCR was performed with primers designed to amplify blaSHV, blaTEM and blaKPC genes. Molecular typing was per...

Abstract The aim of this study was to evaluate the phenotypic and molecular patterns biofilm formation in infection colonization isolates Acinetobacter spp. from patients who were admitted a public hospital Recife-PE-Brazil 2018-2019. For analysis, evaluated by crystal violet staining method; search virulence genes (bap, ompA, epsA, csuE bfmS) performed PCR; ERIC-PCR for typing. Amongst 38 isol...

background: yeast infections are increasing cause of morbidity and mortality in immunocompromised patients. in order to perform a dna-based diagnostic test, availability of a rapid and easy-to-perform dna extraction protocol is essential. in the present study we evaluated colony-pcr as the easiest way to amplification of target dna. methods: instead of using templates of purified genomic dna, w...

objective(s): the nosocomial infections by aspergillus species are associated with constructions and increased dust loads in hospital indoors. our main object was to find the environmental sources of aspergillus species causing hospital acquired infections. materials and methods: the clinical and environmental samplings were performed during 18 months from spring 2010 to summer 2011 in imam edu...

This study aims to evaluate the clinical value of PCR-fluorescent probes for detecting mutation gene associated with extensively drug-resistant tuberculosis (XDR-TB). The molecular species identification 900 sputum specimens was performed using polymerase chain reaction (PCR)–fluorescent probe. mutations drug resistance genes rpoB, katG, inhA, embB, rpsL, rrs, and gyrA were detected. convention...

Background: Malaria is still account for 200 million cases annually. Microscopy is the gold standard technique for malaria parasites detection. PCR-based techniques can detect malaria infections with high sensitivity. The study aimed to evaluate the sensitivity of microscopy technique in the detection of P. falciparum and P. vivax, using monoplex real-time PCR, Gezira State, Central Sudan. Met...