Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established t...

Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims evaluate characteristics semen Limousin bulls different ages, which still used for programs up 12 years age. ages 3, 8, produced in 2021 at Artificial Insemination Center Singosari, East Java Province, ...

Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on...

objective: envenomation by heamotoxic snakes constituted a critical health occurrence in the world. bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. it is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. this study was to evaluate the...

The present study was designed to see whether adding glutathione to tris-citric egg yolk extender improves the quality of buffalo bull spermatozoa stored at 5°C for five days. Semen collected from Nili-Ravi buffalo (Bubalus bubalis) bulls (n = 5) was diluted (37C; 10 x 10 motile spermatozoa per ml) with extender having glutathione (0.0, 0.5, 1.0, 2.0 and 3.0mM). The extended semen was cooled fr...

The use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membrane osmotic water permeability and the stability of protein secondary structure. Here, we describ...

This study was designed to determine the effect of different osmotic pressures on spermatozoa characteristics for cryopreservation of buffalo bull semen using tris egg yolk extender (TEYE). Semen of Nili-Ravi buffalo bulls (n = 04) housed at Semen Production Unit, Qadirabad, Sahiwal, Pakistan, was collected at weekly interval for 10 weeks. Three solutions of tris-citric acid-fructose with osmot...

Diverse treatments, which have been shown by Slayman, C. L. (1977) in Water Relations in Membrane Transport in Plants and Animals (Jungreis, A., Hodges, T. K., Kleinzeller, A., and Schultz, S. G., eds) pp. 69-86, Academic Press, New York, to depolarize the plasma membrane of Neurospora, increase levels of adenosine 3':5'-monophosphate (cyclic AMP) in the organism. The treatments include those p...

An extender has been developed with low-density lipoproteins (LDLs) that eliminates the microbial risks associated with the use of whole egg yolk. The objective of this study was to assess the effects of substituting egg yolk with LDLs for use as an extender in sperm preservation at 4 °C, as well as on spermatozoa motility, plasma membrane and acrosome integrity, at two different concentrations...

The plasma membrane of mammalian cells is susceptible to disruption by mechanical and biochemical damages that frequently occur within tissues. Therefore, efficient and rapid repair of the plasma membrane is essential for maintaining cellular homeostasis and survival. Excessive damage of the plasma membrane and defects in its repair are associated with pathological conditions such as infections...