A method has been described whereby tuberculin P P D is linked to formalinized erythrocytes by a coupling agent, tetrazotized benzidine (1). These antigen-coated erythrocytes are agglutinated by ant ibody to PPD. I t was observed that P P D could inhibit the hemagglutination more effectively than could polysaccharide I. I t appears, therefore, tha t the ant ibody concerned is directed toward th...

OBJECTIVES The diagnosis of typhoid fever is a challenge. Aiming to develop a typhoid diagnostic we measured antibody responses against Salmonella Typhi (S. Typhi) protein antigens and the Vi polysaccharide in a cohort of Bangladeshi febrile patients. METHODS IgM against 12 purified antigens and the Vi polysaccharide was measured by ELISA in plasma from patients with confirmed typhoid fever (...

High-density protein microarrays of recombinant human protein fragments, representing 12,412 unique Ensembl Gene IDs, have here been produced and explored. These protein microarrays were used to analyse antibody off-target interactions, as well as for profiling the human autoantibody repertoire in plasma against the antigens represented by the protein fragments. Affinity-purified polyclonal ant...

When heat-killed whole organisms of Streptococcus mutans strain Ingbritt (serotype c) were injected into rabbits, antibodies to at least 12 antigens were detectable by crossed immunoelectrophoresis. In contrast, when rabbits were immunized with organisms which had been subjected to extraction with the detergent sodium dodecyl sulphate (SDS), antibodies to only two protein antigens were found. T...

Thirty serum samples from clinical cases of flour hypersensitivity were analyzed for wheat or rye flour protein antibodies. The patients included 20 bakers and 10 others who also had occupational flour exposure. Twenty-three cases had antiflour antibodies which recognized antigens other than control sera in the flour protein patterns. The immunologic response of individual cases seemed very var...

1. Repeated large infusions of heterologous plasma proteins can induce a state of specific immunologic unresponsiveness in rabbits. In normal adult rabbits this unresponsiveness in most instances lasts only about as long as the heterologous proteins are detectable in the host (3 to 4 months). In rabbits infused from the time of birth and perhaps x-radiated adult rabbits the induced immunologic ...

To understand the mechanism of Multiple Sclerosis (MS), an autoimmune demyelinating disease, the researchers developed an experimental animal model for MS, which is called EAE (Experimental Allergic Encephalomyelitis). There are several methods for inducing this animal model. In this research the active EAE, which is developed by injecting bovine myelin antigens into genetically susceptibl...

Abstract Humoral immunity is critical for protection against Plasmodium falciparum malaria. In endemic areas, individuals acquire protective malaria through repeated exposure to P. parasites. Understanding these naturally acquired immune responses would be highly informative the development of improved vaccination strategies Antibody uncharacterized protein PF3D7_1136200 correlate with from mal...

Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. The aim of this study was to clone and express the TB'0.4 prot...