mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...

edwardsiella tarda is a versatile pathogen that could survive in different environmental conditions and infect economically important fish species. the whole cell antigenic proteins were extracted from e. tarda and assessed via western blot using anti e. trada rohu and anti e. trada rabbit serum. two strong reacted proteins with antibodies were recovered viz 61 kda and 47 kda from sds page gels...

Introduction. The main biological raw materials for the production of immunobiological preparations identification Bacillus anthracis are its specific antigens, protective antigen and EA1 protein.
 Purpose. To determine antigenic identity immunodominant proteins different genovariants B. isolated by gel chromatography electrophoresis.
 Materials methods. Culture filtrates isogenic var...

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To ...

Background: Streptococcus agalactiae is the main causal pathogen of bovine mastitis (BM), causing considerable economic loss to the dairy industry worldwide. Vaccines against S. agalactiae play an important role in preventing disease. Aims: The aim of this study was to evaluate the immunoprotection of S. agalactiae pilus isla...

background: we have previously reported that a 31 kda protein was screened from the excretory-secretory (es) proteins of tichinella spiralis muscle larvae (ml) by immunoproteomics using early infection sera, and the gene encoding a 31 kda protein from t. spiralis was cloned and expressed in an e. coli expression system. in this study, the recombinant 31 kda antigens were used for detection of a...

Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...

background: dense granule antigens (gra antigens) of toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. previous studies reported expression of amino terminal gra8 protein in fusion with large tags such as glutathione-s-transferase. the present study aimed to produce soluble full length immunogenic gra8 in bacteria. methods: gra8 comple...