A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays posi...

The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NV strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in t...

Objective: To establish the accuracy of computed tomography(CT) chest in diagnosis COVID-19 pneumonia by taking reverse transcriptase-polymerase chain reaction(RT-PCR) as a reference standard and to analyze discordant CT RT-PCR results.Materials Methods: A retrospective cross-sectional study patients presented tertiary health care hospital Punjab, Pakistan for examination with suspicion from Ap...

To quantify hepatitis A virus (HAV) in experimentally contaminated mussels, we developed an internal standard RNA with a 7-nucleotide deletion for competitive reverse transcription (RT)-PCR. Deposited directly into the sample, this standard was used both as extraction control and as quantification tool. After coextraction and competitive RT-PCR, standard and wild-type products were detected by ...

This article describes the use of quantitative PCR for measuring bacterial abundance in environmental samples. The two approaches discussed are: 1) The use of an internal PCR standard constructed to be the same size and have the same sequence as the primary amplification target, but differing from the primary target by 2-3 bases, corresponding to a unique restriction site. This allows the amoun...

BACKGROUND Cytomegalovirus (CMV) infection is the most common cause of congenital infection. Whereas CMV PCR has replaced viral culture and antigen detection in immunocompromised patients because of higher sensitivity, viral culture of neonatal urine is still referred to as the gold standard in the diagnosis of congenital CMV infection. OBJECTIVE To compare real-time CMV PCR with shell vial c...

Abstract Background Real-time Reverse transcriptase—polymerase chain reaction (RT-PCR) is a regular technique for detecting SARS-CoV-2 infection. Human RNase P sequence using as internal control PCR assay usually. This article discusses the correlation between result and human gene result. Methods Nasopharyngeal swabs were collected from two patients within three consecutive days. We detected t...

Background: Neoadjuvant chemotherapy has become the standard treatment for patients with locally advanced breast cancer. However, hormone receptor positive (especially human epidermal growth 2 negative) cancer show low response rate to neoadjuvant chemotherapy. Whether chemo-endocrine therapy (NCET) can improve pathological complete (pCR) of these remains controversial. Methods: A systematic li...

A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which the same primers are used. The amount of mRNA is then quantitated by extrapolating against the standard curve generated with the internal stan...