Brown spot caused by Bipolaris oryzae is an important rice disease in Southern coast of Caspian Sea, the major rice growing region in Iran. A total of 45 Trichoderma isolates were obtained from rice paddy fields in Golestan and Mazandaran provinces which belonged to Trichoderma harzianum, T. virens and T. atroviride species. Initially, they were screened against B. oryzae by antagonism tests in...

This study aimed to determine the effect of biochar and Trichoderma harzianum toward lead removal in soil, absorption, content plant tissue also growth yield shallot cultivated on lead-contaminated soil. The experimental design used was a completely factorial randomized block consisting 2 factors. first factor corn cobs which applied 1 week after basic fertilizer treatment consisted 4 levels, n...

A simple detached leaf-droplet inoculation bioassay was devised in order to investigate the potential for biological control of Botrytis cinerea by indigenous Trichoderma harzianum isolates. Following preliminary in vitro screening of the antagonistic capacity of four local isolates of Trichoderma harzianum (T1-T4) against B. cinerea, T3 spore suspensions were then tested for their ability to c...

A gene that encodes an extracellular aspartyl protease from Trichoderma harzianum CECT 2413, papA, has been isolated and characterized. Based on several conserved regions of other fungal acid proteases, primers were designed to amplify a probe that was used to isolate the papA gene from a genomic library of T. harzianum. papA was an intronless ORF which encoded a polypeptide of 404 aa, includin...

Many root diseases can cause serious damage to tomato (Solanum lycopersicum L.) production during the seedling growth period, particularly Rhizoctonia solani, Pythium spp., and Alternaria spp. Paclobutrazol (PBZ) has proved reduce of blight disease on seedlings. The present investigation was designated evaluate infected seedlings with R. solani under applications Trichoderma harzianum alone, PB...

Cassava root rot causes significant production losses. Difficulties of management, along with the lack chemical fungicides officially registered by Ministry Agriculture, Livestock and Supply (MAPA), require alternative control methods. This study investigated in vitro antagonistic activity Trichoderma harzianum as well a biological fertilizer MICROGEO® on Fusarium solani. The phytophatogenic st...

This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) me...